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We narrowed to 2,699 results for: dap

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Showing: 2121 - 2140 of 2699 results

  2. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

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  3. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  4. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  8. RHO3

    Bacterial Strain
    #124700
    Purpose
    E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.
    Depositor
    Herbert Schweizer
    Article
    Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):
    Bacterial Resistance
    DAP (400 µg/ml)
    Available Since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only

  11. pBP-P_phlF-PPhlF

    Plasmid
    #204075
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    phlF repressor and PPhlF DAPG-inducible promoter
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204075-plasmid-map-sequence-id-413890

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  12. pBP-P_TALEsp1_PUPsp1-phlF_PPhlF

    Plasmid
    #204080
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    TALEsp1-stabilized promoter driving phlF repressor; PPhlF DAPG-inducible promoter
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204080-plasmid-map-sequence-id-413895

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  13. mr-GST full length

    Plasmid
    #112982
    Purpose
    For protein expression and purification of full-length Drosophila mr (APC2)
    Depositor
    Terry Orr-Weaver
    Insert
    mr (APC2) (mr Fly)
    Expression
    Bacterial
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pcDNA3-TRAM-CFP

    Plasmid
    #13027
    Depositor
    Doug Golenbock
    Insert
    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    Tags
    CFP
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13027-plasmid-map-sequence-id-7425

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  15. pOpen-K12polLF (exo-)

    Plasmid
    #165522
    Purpose
    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    Use
    Synthetic Biology
    Available Since
    Dec. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165522-plasmid-map-sequence-id-350938

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  16. hPLD3-sgRNA-Cas9_mcherry

    Plasmid
    #199342
    Purpose
    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199342-plasmid-map-sequence-id-390619

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  17. hPLD4-sgRNA-Cas9_mcherry

    Plasmid
    #199343
    Purpose
    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    sgRNA: accagtagtatgaagccacg
    Promoter
    human U6
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199343-plasmid-map-sequence-id-390620

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  19. mr-GST N-term fusion

    Plasmid
    #112983
    Purpose
    For protein expression and purification of N-terminus of Drosophila mr (APC2)
    Depositor
    Terry Orr-Weaver
    Insert
    mr (APC2) (mr Fly)
    Expression
    Bacterial
    Mutation
    N-term only
    Available Since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112983-plasmid-map-sequence-id-217464

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  20. AbVec_IGHC

    Plasmid
    #183701
    Purpose
    Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.
    Depositor
    David Nemazee
    Insert
    IGHG1 (IGHG1 Human)
    Expression
    Mammalian
    Promoter
    SP6
    Available Since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183701-plasmid-map-sequence-id-358532

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Showing: 2121 - 2140 of 2699 results