We narrowed to 19,950 results for: form

1. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mScarlet-Rab9aS21N

   Plasmid

   #169073

   Purpose

   Express human dominant negative form of Rab9a(S21N) in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rab9a (RAB9A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDAI-SceI

   Plasmid

   #32061

   Purpose

   Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

   Depositor

   Miguel Valvano

   Article

   Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

   Insert

   I-SceI nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pdhfr

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSANG10-3F

   Plasmid

   #39264

   Depositor

   John McCafferty

   Article

   Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

   Type

   Empty backbone

   Use

   Bacterial expression, bacterial expression of re…

   Tags

   6xHis, pelB leader, and tri-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-SFFV-ZFYVE27-WPRE-UbC-mCherry

   Plasmid

   #214470

   Purpose

   Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   ZFYVE27 (ZFYVE27 Human)
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA-ISceID44A

   Plasmid

   #59424

   Purpose

   Expresses a catalytically inactive form of the endonuclease ISceI

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   endonuclease ISceI mutant (I-SceI )
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   changed aspartate 44 to alanine (D44A)

   Promoter

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET24c(+)-mtEFTu

   Plasmid

   #30535

   Depositor

   Linda Spremulli

   Article

   Woriax et al Biochim Biophys Acta. 1995 Dec 27. 1264(

   Insert

   Mature bovine mitochondrial elongation factor Tu
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Mature form amino acids 44-452. Mito import seque…

   Promoter

   Available since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pET3C-ARL16(CO)-His6

   Plasmid

   #216485

   Purpose

   Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.

   Depositor

   Richard Kahn

   Insert

   ADP-ribosylation factor-like 16 (ARL16 Human)
   

   Use

   Tags

   includes GSGSGSHHHHHH (His6) tag at C-terminus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSG5 E107K T Ag

   Plasmid

   #133710

   Purpose

   Large T antigen mutant form in pSG5 vector for mammalian expression.

   Depositor

   James A. DeCaprio

   Article

   Zalvide et al Mol Cell Biol. 1995 Oct . 15(10):5800-10

   Insert

   SV40gp6 (SV40gp6 SV40)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E107K

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK370

    Plasmid

    #73595

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    

    Use

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Silent mutation in Arg299 codon.

    Promoter

    GAL1 and GAL10

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2-Tm2AG

    Plasmid

    #26773

    Depositor

    Shankar Srinivas

    Article

    Trichas et al BMC Biol. 2008 . 6():40.

    Insert

    TdTomato-2A-H2BGFP-SV40pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutation in 2A peptide that renders it non-functi…

    Promoter

    Available since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3- myc NES-mCE(K294A) NLS

    Plasmid

    #82467

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NLS deleted from mouse Capping Enzyme (Rngtt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of KRKY from 573-657

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EGFP-alphasynuclein-A53T

    Plasmid

    #40823

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A53T (aggregates more rapidly than the wild-type …

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hGip-R

    Plasmid

    #14942

    Depositor

    Bernard Thorens

    Article

    Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

    Insert

    Gip-R (GIPR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Short form of the receptor

    Promoter

    Available since

    June 7, 2007

    Availability

    Academic Institutions and Nonprofits only

15. 6599-SUMO1GG

    Plasmid

    #13377

    Depositor

    Anne Dejean

    Article

    Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mature form ending with GG

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET11a-SUMO1

    Plasmid

    #53138

    Purpose

    E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-97)

    Promoter

    T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTXB1-EXO1b

    Plasmid

    #68267

    Purpose

    Bacterial expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Use

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    T7

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pTrex-n-eGFP

    Plasmid

    #62544

    Purpose

    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome

    Depositor

    Rick Tarleton

    Article

    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

    Insert

    eGFP
    

    Use

    (1)trypanosoma cruzi expression of egfp (2) genom…

    Tags

    None

    Expression

    Mutation

    None

    Promoter

    Ribo-HX1

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-BiP-Myc-KDEL T37G

    Plasmid

    #27165

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Use

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    ATP bound form: T37G

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pET3a RegIIIg

    Plasmid

    #64936

    Purpose

    bacterial expression of processed mouse RegIIIgamma

    Depositor

    Lora Hooper

    Article

    Mukherjee et al J Biol Chem. 2009 Feb 20;284(8):4881-8.

    Insert

    RegIIIg (Reg3g Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    processed form

    Promoter

    T7

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only