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pASKt15C+SrtA2

Plasmid

#64984

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET3a pro-RegIIIg

Plasmid

#64934

Purpose

bacterial expression of full length mouse RegIIIgamma

Depositor

Lora Hooper

Article

Cash et al Protein Expr Purif. 2006 Jul;48(1):151-9

Insert

RegIIIg (Reg3g Mouse)

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pASKt15C+SrtAwoH

Plasmid

#65017

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pACYC_ZmSAE2-FLAG

Plasmid

#91943

Purpose

Bacterial expression of maize SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

ZmSAE2-FLAG

Tags

FLAG

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pPhlF-BCD20-Citrine-ssrA P64U23C88T18 v1-1 (586)

Plasmid

#70743

Purpose

for building 3-color reporter plasmid

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine-ssrA

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE65

Plasmid

#79447

Purpose

recipient plasmid [single nuclease]; pPcUbi:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE146

Plasmid

#79449

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE167

Plasmid

#79457

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE168

Plasmid

#79458

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Smad4 P130S

Plasmid

#80892

Purpose

mammalian expression of Smad4 P130S

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

SMAD4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

colorectal cancer mutation P130S; additional D2E

Promoter

CMV

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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