We narrowed to 63,495 results for: ANG

1. pTT5-hSDC1-CS2

   Plasmid

   #52367

   Purpose

   expresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Expression

   Mammalian

   Mutation

   R95Q and S194A (according to numbering in Fig. 2A…

   Available Since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-flex-taCasp3-TEVp

   Plasmid

   #45580

   Depositor

   Jim Wells, Nirao Shah

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Has Service

   AAV1 and AAV5

   Inserts

   Caspase 3 (CASP3 Synthetic, Human)
   Tobacco Etch Virus protease
   

   Use

   AAV; Adeno associated viral vector

   Mutation

   Linker replaced with a TEV protease cleavage site

   Promoter

   EF1a

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-MEKDD

   Plasmid

   #31202

   Purpose

   Gateway entry vector containing MEKDD.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   MAPKK1 (MAP2K1 Human)
   

   Use

   Gateway donor vector

   Mutation

   S218D S222D

   Available Since

   June 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. cryS96

   Plasmid

   #67519

   Purpose

   αB-crystallin minipeptide fused to ELP (S96).

   Depositor

   Andrew MacKay

   Article

   Wang et al J Control Release. 2014 Oct 10;191:4-14.

   Insert

   mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)
   

   Tags

   elastin-like polypeptide (ELP) S96

   Expression

   Bacterial

   Mutation

   mini-αB-crystallin contains residues 73–92 of aB-…

   Promoter

   T7

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pTT5-hSDC1-CS1/CS2

   Plasmid

   #52370

   Purpose

   expresses human Syndecan-1 point mutant at S184A and S194A in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Expression

   Mammalian

   Mutation

   R95Q, S184A and S194A (according to numbering in …

   Promoter

   CMV

   Available Since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pTT5-hSDC1-CS1

   Plasmid

   #52365

   Purpose

   expresses human Syndecan-1 point mutant at S184A in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Expression

   Mammalian

   Mutation

   R95Q and S184A (according to numbering in Fig. 2A…

   Promoter

   CMV

   Available Since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pTT5-hSDC1-S15A

   Plasmid

   #52363

   Purpose

   expresses human Syndecan-1 serine 15 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Expression

   Mammalian

   Mutation

   S15A and R95Q (according to numbering in Fig. 2A …

   Promoter

   CMV

   Available Since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

8. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60229

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. LSL-Cas9-Rosa26TV

   Plasmid

   #61408

   Purpose

   Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   3xFLAG and P2A

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CAG

   Available Since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60230

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Promoter

    U6 and hSyn

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60228

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60227

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60225

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-K-RAS V12

    Plasmid

    #31200

    Purpose

    Gateway donor vector containing KRAS G12V.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    K-Ras (KRAS Human)
    

    Use

    Gateway donor vector

    Mutation

    G12V constitutively active

    Available Since

    June 24, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pDONR223-H-RAS V12

    Plasmid

    #31201

    Purpose

    Gateway donor vector containing HRAS G12V.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    H-Ras (HRAS Human)
    

    Use

    Gateway donor vector

    Mutation

    G12V constitutively active

    Available Since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pTT5-hSDC1-S15/25A

    Plasmid

    #52364

    Purpose

    expresses human Syndecan-1 serine 15 and 25 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    S15A, S25A and R95Q (according to numbering in Fi…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTT5-hSDC1-N20

    Plasmid

    #52355

    Purpose

    expresses human syndecan 1 'del 1- 20' truncation mutant in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of SDC1 aa2-21 (according to numbering i…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pTT5-hSDC1-2

    Plasmid

    #52378

    Purpose

    expresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    GAGAL of syndecan-1 replaced with of GADED of SDC…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pTT5-hSDC1-4

    Plasmid

    #52375

    Purpose

    expresses human Syndecan-1 GAGAL replaced with GDLDD of SDC4 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    GAGAL of syndecan-1 replaced with GDLDD of SDC4; …

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

20. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60231

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Promoter

    U6 and hSyn

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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