We narrowed to 8,842 results for: Tet-on
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Plasmid#186710PurposeN-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.DepositorInsertalr4766
UseSynthetic BiologyTagsHis-Tag (6His)PromoterPtrc.x.tetO2Available SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo
Plasmid#179440PurposeExpresses rTetR-HDAC4(human) for gene silencing, integration by PiggybacDepositorInsertH2B-mIFP-T2A-rTetR-HDAC4
ExpressionMammalianPromoterpGKAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-EnR-MCS_N-term
Plasmid#136884PurposeDoxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-VP64-MCS_N-term
Plasmid#136886PurposeDoxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertVP64 activator domain
UseLentiviralTagsFlagExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-KRAB-MCS_N-term
Plasmid#136888PurposeDoxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertKRAB repressor domain
UseLentiviralTagsFlagExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)
Plasmid#160570PurposeA version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffoldDepositorInsertsgRNA:scaffold tetraloop MS2 aptamer SAM
UseCRISPRMutationBsaI and BsmBI sites removedAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA
Plasmid#121804PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETaDepositorInsertHA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA
Plasmid#121808PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETaDepositorInsertHA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-L900A(pL91)
Plasmid#124395PurposeE.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-L900A (TET1 Human)
TagsGB1-HistidineExpressionBacterialMutationL900AAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-T898E(pL91)
Plasmid#124394PurposeE.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-T898E (TET1 Human)
TagsGB1-HistidineExpressionBacterialMutationT898EAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-T898A(pL91)
Plasmid#124393PurposeE.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-T898A (TET1 Human)
TagsGB1-HistidineExpressionBacterialMutationT898AAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-L897A(pL91)
Plasmid#124392PurposeE.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-L897A (TET1 Human)
TagsGB1-HistidineExpressionBacterialMutationL897AAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-I894A(pL91)
Plasmid#124391PurposeE.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-I894A (TET1 Human)
TagsGB1-HistidineExpressionBacterialMutationI894AAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)
Plasmid#91874PurposeRapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2DepositorInsertRapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)
TagsmCherryExpressionBacterial and MammalianAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)
Plasmid#70733Purposeplasmid for building 5n1DepositorInserttetR-ssrA
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)
Plasmid#70739Purposeplasmid for building 5n2DepositorInserttetR-ssrA
Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
Plasmid#221235PurposeTet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.DepositorInsertTadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
UseLentiviralAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacExpressionMammalianPromoterEF1AAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69546PurposeTet-On system expressing MyoD-2A-GFP-2xNLS.DepositorInsertsUseLentiviralTags2x nuclear localization sequence and T2A "sk…MutationRemoved stop codon to fuse it to the 2A-eGFPnls i…Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-off Full length Tau (0N4R, P301L/V337M)
Plasmid#188573PurposeDox-repressible expression of the 0N4R Tau isoformDepositorInsert0N4R Tau (MAPT Human)
UseLentiviralExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only