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linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF2

Plasmid

#241193

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF1

Plasmid

#241192

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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T2A-mCherry-P2A-NTRv2-pA-ORF-1

Plasmid

#241196

Purpose

Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry; NTR-v2

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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T2A-mCherry-P2A-NTRv2-pA-ORF-2

Plasmid

#241197

Purpose

Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry; NTR-v2

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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SA-EGFP-pA

Plasmid

#241211

Purpose

Middle entry vector for mini-Golden subcloning platform; eGFP

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-lifeact-mNeongreen-ORF-3

Plasmid

#241174

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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memb-mNeongreen

Plasmid

#241175

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mNeongreen-ORF1

Plasmid

#241176

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mNeongreen-ORF2

Plasmid

#241177

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
linker-P2A-mNeongreen-ORF3

Plasmid

#241178

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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FRT-acrymCherry-FRT

Plasmid

#241179

Purpose

Middle entry vector for mini-Golden subcloning platform; acry-mCherry

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

acry-mCherry

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF1

Plasmid

#241180

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF2

Plasmid

#241181

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_mCherry-pA

Plasmid

#200521

Purpose

Mini circle middle entry plasmid for mCherry- pA with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_BFP-pA

Plasmid

#200533

Purpose

mini circle middle entry plasmid for BFP-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_EGFP-pA

Plasmid

#200513

Purpose

Mini circle middle entry plasmid for GFP-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP-pA

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-Spe-KpnI-ubb-nmKate

Plasmid

#238373

Purpose

Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

Depositor

Juan Manuel González-Rosa

Publication

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nmKate

Promoter

ubiquitin

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-A

Plasmid

#221713

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D683A in active site

Promoter

simian CMV IE94

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2Y-F

Plasmid

#221715

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Y680F in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-E

Plasmid

#221714

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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