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pAG426GAL-mRuby3

Plasmid

#166144

Purpose

mRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Expression

Yeast

Promoter

GAL1

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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ACE2(-202)-luc

Plasmid

#31112

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -202 to +103

Available Since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pcmv3-STING-C88S-FLAG

Plasmid

#198187

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C88S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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MSCVpuro-6xHis_SUMO2

Plasmid

#164938

Purpose

Expresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Small Ubiquitin Like Modifier 2 (SUMO2 Human)

Use

Retroviral

Tags

6XHIS

Promoter

PGK promoter

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF1419

Plasmid

#143112

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

VDR (VAX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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hFLT1 delta CTD

Plasmid

#86036

Purpose

human VEGFR1 with deletion of the entire intracellular domain

Depositor

Christie Thomas

Article

Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi

Insert

human Flt1 delta CTD (FLT1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

deta CTD

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pH3R-P2A-mCherry-N1

Plasmid

#84332

Purpose

Co-expresses (untagged) Histamine-3 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H3R-P2A-mCherry (HRH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRD421

Plasmid

#163112

Purpose

Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd

Tags

His-tag with TEV-cleavable linker

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-H159Q-HA

Plasmid

#198183

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) H159Q

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Tags

HA

Expression

Mammalian

Mutation

The histidine in IRG1 at position 159 mutates to …

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pF711-pET28a-Hs-delta25-MCAD-NHis wt

Plasmid

#85112

Purpose

expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta25) MCAD (ACADM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_NoCys-Myc

Plasmid

#201169

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Mutation

C39A, C57A, C77A, C122A, C265A, C192A

Promoter

MESV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_fibrillarin

Plasmid

#65709

Purpose

Expresses CNL-fibrillarin in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

fibrillarin (FBL Human)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pONL-C1_Rab11a

Plasmid

#65707

Purpose

Expresses ONL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-ONL-CP

Plasmid

#65716

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST

Use

Luciferase

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-YNL-CP

Plasmid

#65714

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST

Use

Luciferase

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-CNL-CP

Plasmid

#65715

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST

Use

Luciferase

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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FusionRed-Dectin1A-N-10

Plasmid

#56112

Purpose

Localization: Membrane, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Dectin1A (CLEC7A Human)

Tags

FusionRed

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pT2-7xTcf-NLS-YNL

Plasmid

#65711

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, YNL

Use

Luciferase; Tol2 system

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_Rab11a

Plasmid

#65706

Purpose

Expresses CNL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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Ntng1.c-Fc-His

Plasmid

#72112

Purpose

Expresses the extracellular region of the Netrin G1, isoform c protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntng1.c (Ntng1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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