We narrowed to 4,523 results for: ARA-2

1. CRY2low-mCherry-Raf1

   Plasmid

   #104066

   Purpose

   Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2low-mCherry-Raf1 (CRY2, RAF1 Mustard Weed, Human)
   

   Expression

   Mammalian

   Mutation

   CRY2 aa 1-491 truncation, R489E A491D

   Promoter

   CMV

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-BLAST flag-CAD T456A S1406A

   Plasmid

   #169823

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   T456A S1406A; TCCC -> AGTC silent mutations at…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-BLAST flag-CAD T456A S1859A

   Plasmid

   #169824

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   T456A S1859A; TCCC -> AGTC silent mutations at…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPHR-EYFP-hGCN5mut

   Plasmid

   #103828

   Purpose

   Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-hGCN5mut (CRY2, KAT2A Mustard Weed, Human)
   

   Expression

   Mammalian

   Mutation

   hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

   Promoter

   CMV

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPHR-EYFP-hGCN5-NLS

   Plasmid

   #103827

   Purpose

   Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-hGCN5-NLS (CRY2, KAT2A Mustard Weed, Human)
   

   Expression

   Mammalian

   Mutation

   hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

   Promoter

   CMV

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPHR-EYFP-hGCN5

   Plasmid

   #103826

   Purpose

   Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-hGCN5 (CRY2, KAT2A Mustard Weed, Human)
   

   Expression

   Mammalian

   Mutation

   hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

   Promoter

   CMV

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 8024 GFP_Kras_G12C_2A_puro

   Plasmid

   #64373

   Purpose

   This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   mouse Kras2A (Kras Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Mutation

   changed glycine 12 to cysteine

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 8027_GFP-KrasG12C_2B_puro

   Plasmid

   #64372

   Purpose

   This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   mouse Kras2B (Kras Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Mutation

   changed glycine 12 to cysteine

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 8798 GFP-KrasG12C_2B_hygro

   Plasmid

   #64376

   Purpose

   This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   mouse Kras2B (Kras Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Mutation

   changed glycine 12 to cysteine

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 8047_GFP-Kras2B_ires_puro

    Plasmid

    #64371

    Purpose

    This a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    mouse Kras2B (Kras Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Mutation

    none

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPHR-mCherry-FUSN-mHP1alpha

    Plasmid

    #181892

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-FUSN-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-GSDMD_1-275

    Plasmid

    #218230

    Purpose

    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 1-275

    Available Since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pPHR-mCherry-PGL-1-mHP1alpha

    Plasmid

    #181894

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPHR-mCherry-nE-mHP1alpha

    Plasmid

    #181890

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDTnLAPP2A frame 0

    Plasmid

    #28226

    Depositor

    Harald von Melchner

    Article

    Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

    Insert

    Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
    

    Use

    Retroviral

    Tags

    EGFP and nLAP-tag

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDTnLAPP2A frame 1

    Plasmid

    #28227

    Depositor

    Harald von Melchner

    Article

    Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

    Insert

    Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein
    

    Use

    Retroviral

    Tags

    EGFP and nLAP-tag

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPHR-mCherry-RGG-GFP-RGG-mHP1alpha

    Plasmid

    #181893

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid

    #181891

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mouse, Mustard Weed)
    

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAM-35s-ABI2-YFPn-1

    Plasmid

    #102383

    Purpose

    split YFP. Plant expression of ABI2-YFPn-1

    Depositor

    Silke Robatzek

    Insert

    ABI2 (ABI2 Mustard Weed)
    

    Tags

    YFPn

    Expression

    Plant

    Promoter

    35S

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. YIp204-PADH1-AFB2

    Plasmid

    #99531

    Purpose

    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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