We narrowed to 4,755 results for: MLS

1. CCND2 targeting gRNA

   Plasmid

   #215318

   Purpose

   Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CCND1 targeting gRNA

   Plasmid

   #215153

   Purpose

   Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pD649-HAsp-LCN2-COMP5AP-AviTag-9xHis

   Plasmid

   #157141

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   LCN2 (LCN2 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)

   Plasmid

   #208951

   Purpose

   A variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   TGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)

   Plasmid

   #208950

   Purpose

   A variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   FBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)

   Plasmid

   #208949

   Purpose

   A variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   RepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HUHe variant click editor (CE1) - pCMV-T7-TraI-nCas9-EcKlenow (JO604)

   Plasmid

   #208948

   Purpose

   A variant CE1 construct with TraI HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   TraI-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SITS-MmCav1-EGFP-APEX2

   Plasmid

   #170525

   Purpose

   Vector expression of MmCav1-EGFP-APEX2 in Leishmania cell free system. Parton lab clone JEJ

   Depositor

   Rob Parton

   Article

   Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

   Insert

   Caveolin1 (Cav1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   APEX2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FokI(inactive)-mCherry-LacI-NLS

   Plasmid

   #215100

   Purpose

   Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   FokI(inactive)-mCherry-LacI-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   FokI(inactive) mutant D69A.

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pD649-HAsp-TNFRSF6B-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156713

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TNFRSF6B (TNFRSF6B Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Aldh1L1 scFv [N103/31]

    Plasmid

    #182080

    Purpose

    Mammalian Expression of Aldh1L1 scFv. Derived from hybridoma N103/31.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Aldh1L1 (Rattus norvegicus) (Aldh1l1 Mouse)
    

    Use

    Tags

    Sortase, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Anti-Beta4-spectrin [N393/76R]

    Plasmid

    #177542

    Purpose

    Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/76.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Anti-TrpV1 [N221/17R]

    Plasmid

    #177517

    Purpose

    Mammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/17.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Anti-nNOS/NOS1 [L121/133R]

    Plasmid

    #188170

    Purpose

    Mammalian Expression Plasmid of anti-nNOS/NOS1 (Human). Derived from hybridoma L121/133

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-nNOS/NOS1 (Homo sapiens) recombinant mouse monoclonal antibody (NOS1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Anti-EAAC1 [N420/24R]

    Plasmid

    #206572

    Purpose

    Mammalian Expression Plasmid of anti-EAAC1 (Rat). Derived from hybridoma N420/24.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-EAAC1 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc1a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Anti-EAAC1 [N180/41R]

    Plasmid

    #206615

    Purpose

    Mammalian Expression Plasmid of anti-EAAC1 (Rat). Derived from hybridoma N180/41.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-EAAC1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc1a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pD649-HAsp-HLA-F-COMP5AP-AviTag-9xHis

    Plasmid

    #157398

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    HLA-F (HLA-F Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pD649-HAsp-SLAMF1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156642

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SLAMF1 (SLAMF1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pD649-HAsp-KIRREL2-COMP5AP-AviTag-9xHis

    Plasmid

    #157444

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIRREL2 (KIRREL2 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pD649-HAsp-ISLR-COMP5AP-AviTag-9xHis

    Plasmid

    #157408

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    ISLR (ISLR Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge