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pJW4.27

Plasmid

#62877

Purpose

Bacterial expression of MBP-MEG-1 S574A

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MEG-1 S574A

Tags

MBP

Expression

Bacterial

Mutation

S574A

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAD-g6

Plasmid

#61497

Purpose

For expression of C. briggsae gld-1, used for rescue of C. elegans q485 homozygotes

Depositor

Eric Haag

Article

Beadell et al Proc Natl Acad Sci U S A. 2011 Dec 6;108

Insert

gld-1 (Cbr-gld-1 Caenorhabditis briggsae)

Tags

FLAG and His

Expression

Worm

Promoter

gld1

Available Since

Feb. 25, 2015

Availability

Academic Institutions and Nonprofits only
pUC19 gene 60 thru gap + Cys

Plasmid

#49161

Purpose

Template for generating gene 60 mRNA truncated two codons after coding gap + Cys

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Mutation

gene 60 mRNA truncated two codons after coding ga…

Promoter

T7

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1-C103A

Plasmid

#58419

Purpose

Expresses murine IFITM1-C103A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 103 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C83A

Plasmid

#58418

Purpose

Expresses murine IFITM1-C83A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 83 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C50A

Plasmid

#58417

Purpose

Expresses murine IFITM1-C50A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 50 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C49A

Plasmid

#58416

Purpose

Expresses murine IFITM1-C49A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 49 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
Syntaxin-4 M2 (Y115A, Y148A, Y251A)

Plasmid

#44030

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Tags

2xMyc and His

Expression

Mammalian

Mutation

Y115A Y148A Y251A

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
pTH729-minRLuc

Plasmid

#38225

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Mutation

Every codon of the Renilla coding sequence was ex…

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTH738-CENBEVYslow

Plasmid

#38220

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

GCN4-based single uORF-containing 5'-UTR

Expression

Yeast

Mutation

The start codons of uORFs2, 3 and4 of the yeast G…

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pHL1324

Plasmid

#37638

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
TetR
CsrA

Use

Synthetic Biology

Expression

Bacterial

Promoter

pCon and pConNoHind

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only
PCDNA3 T7 DLC2

Plasmid

#24268

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Tags

T7

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag BimEL (3SA)

Plasmid

#23100

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (3SA) (Bcl2l11 Mouse)

Tags

GST

Expression

Bacterial

Mutation

changed Serine 55/65/73 to alanine

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pBacPAK-HFT-dKDM4A-H195A

Plasmid

#20236

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Mutation

changed His 195 to Ala

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron4_sg1_pX458

Plasmid

#127175

Purpose

sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 4 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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HIVGKO

Plasmid

#112234

Purpose

HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

Depositor

Eric Verdin

Article

Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

Insert

csGFP-EF1a-mKO2

Use

Lentiviral

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-PELP1

Plasmid

#65208

Purpose

Full length mammalian expression vector for hPELP1

Depositor

Ratna Vadlamudi

Article

Nagpal et al Mol Cancer Res. 2008 May;6(5):851-61. do

Insert

PELP1 (PELP1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only
myc-Rictor

Plasmid

#1860

Purpose

Please see Addgene item #11367 for an updated version of this plasmid.

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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