We narrowed to 4,154 results for: pCMV

1. pCMV-FLAG-WRAP53beta-siRNA-resistant

   Plasmid

   #64680

   Purpose

   expresses siRNA resistant WRAP53beta

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   WD40-encoding RNA antisense to P53 (WRAP53 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   siRNA resistant

   Promoter

   CMV

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-myc-Rem2 RNAiR2 short

   Plasmid

   #51596

   Purpose

   expresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2 Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   silent mutations at shRNA 1 and 2 recognition sit…

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-myc-Rem2 R236A/L263A

   Plasmid

   #51589

   Purpose

   expresses RNAiR myc-tagged Rem2 non-Ca channel interacting

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R236A and L263A; silent mutations at shRNA 1 and …

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-myc-Rem2 S69A/S334A

   Plasmid

   #51588

   Purpose

   expresses RNAiR myc-tagged Rem2 non-14-3-3 interacting

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S69A and S334A; silent mutations at shRNA 1 and 2…

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Flag-YAP-S127A/G385V

   Plasmid

   #33072

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S127A, G385V

   Promoter

   CMV

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-Flag-YAP-S127/D383A

   Plasmid

   #33078

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S127A, D383A

   Promoter

   CMV

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pCMV Ub Lex VP16 (F442A) HA (P#1711)

   Plasmid

   #14596

   Depositor

   Matija Peterlin

   Article

   Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

   Insert

   LexA
   

   Use

   Tags

   HA, Ubiquitin, and VP16 (F442A)

   Expression

   Mammalian

   Mutation

   The VP16 sequence contains an F442A mutation. The…

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

   Plasmid

   #115141

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy, Keith Joung

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Orf2mor anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

   Plasmid

   #221225

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA M6-UGI-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-AID-BLM-UGI-T2A-mTagBFP2

    Plasmid

    #221222

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-BLM-UGI-T2A-mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

    Plasmid

    #234826

    Purpose

    STITCHR R2Tocc editor

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    nCas9h840a-xten-R2Tocc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

    Plasmid

    #214102

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid

    #185910

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid

    #185914

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

    Plasmid

    #223087

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid

    #185911

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158990

    Purpose

    Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid

    #185919

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge