Addgene

We narrowed to 31,294 results for: Eng

Showing: 301 - 330 of 31294 results

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pMST-1313

Plasmid

#118076

Purpose

pTEF1-cexA-tCYC1

Depositor

Michael Sauer

Article

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

pTEF1-cexA-tCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

pTEF1

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPS3911

Plasmid

#113330

Purpose

control for Hermes transposition; TIR-flanked LEU2, transposase absent

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Insert

beta-isopropyl malate dehydrogenase

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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JBEI-9348

Plasmid

#84981

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

PMD-NudB

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-9314

Plasmid

#84979

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

PMD

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PHBA01

Plasmid

#83535

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC gene

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-UBiC-CYC1t

Use

Tags

Expression

Yeast

Mutation

Promoter

pTEF1

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDS2-aurZ

Plasmid

#70151

Purpose

Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

aurZ

Use

Tags

Expression

Yeast

Mutation

Promoter

Tef

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBbA2k-RFP

Plasmid

#35327

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA2c-RFP

Plasmid

#35326

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pSLQ6332 - ACTB CRISPRa CLIP Donor

Plasmid

#210015

Purpose

CLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

dLbCpf1-miniVPR-T2A-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMSP2N2

Plasmid

#29520

Depositor

Stephen Sligar

Article

Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

Insert

MSP2N2

Use

Tags

7-His tag

Expression

Bacterial

Mutation

tandem MSP; His-tag on N-terminus followed by spa…

Promoter

Available since

May 3, 2011

Availability

Academic Institutions and Nonprofits only

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PBAD-his6-prkA-pACYC184

Plasmid

#41041

Depositor

Ichiro Matsumura

Article

Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

Insert

prkA

Use

Tags

6xHis and T7 epitope tag

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pGRB

Plasmid

#71539

Purpose

for expression of gRNA in E. coli

Depositor

Tao Chen

Article

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

J231196

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GFP

Plasmid

#158130

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS2c-RFP

Plasmid

#35329

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT3928

Plasmid

#113331

Purpose

control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Inserts

beta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pTS1106_Tier3(Lenti)

Plasmid

#169661

Purpose

Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction (PCMV-5'LTR-Psi-gag-env-A1::A2::A3-WPRE-3'LTR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b RNase A

Plasmid

#58903

Purpose

Bacterial expression of bovine pancreatic ribonuclease A

Depositor

Ronald Raines

Article

delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Insert

RNase A (RNASE1 Bovine)

Use

Tags

pelB sequence

Expression

Bacterial

Mutation

See Depositor Comments below

Promoter

T7

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pBbE0k-RFP

Plasmid

#35365

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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p276 eSpCas9_2gRNAs_hH11

Plasmid

#164850

Purpose

gRNA vector for targeting human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

gRNAs for targeting human H11 locus

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBbB5c-GFP

Plasmid

#35353

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pgRNA

Plasmid

#215560

Purpose

Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

gRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbE1k-DDC

Plasmid

#71723

Purpose

dopa decarboxylase from pig

Depositor

Taek Soon Lee

Article

Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

Insert

dopa decarboxylase

Use

Tags

Expression

Mutation

Promoter

pTrc

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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rescue plasmid

Plasmid

#135971

Purpose

expresses copGFP and puromycin resistance genes

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbB6c-GFP

Plasmid

#35356

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

LlacO-1

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbB8c-GFP

Plasmid

#35362

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BAD

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbB0c-RFP

Plasmid

#35366

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVEX-SNAP-GFP

Plasmid

#46846

Purpose

SNAP-GFP fusion

Depositor

Florian Hollfelder

Article

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Insert

SNAP-GFP fusion

Use

In vitro expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbB8k-GFP

Plasmid

#35363

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BAD

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA6c-RFP

Plasmid

#35290

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

LlacO-1

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbE1a-RFP

Plasmid

#35331

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Trc

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge