-
Plasmid#111492PurposeMet-tRNA formyltransferaseDepositorInsertMet-tRNA formyltransferase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A,M142A(KaganF62)
Plasmid#52002PurposeExpresses the human MAVS CDS containing two point mutations M1A and M142ADepositorInsertMAVS
UseTagsExpressionMammalianMutationchanged Met 1 to Ala and Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A,M142A-invitro(KaganE14)
Plasmid#52018PurposeUsed for in vitro expression of the human MAVS CDS containing the point mutations M1A,M142ADepositorInsertMAVS-M1A,M142A
UseTagsExpressionMammalianMutationchanged Met 1 to Ala and Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-MAVS-M1A,M142A(KaganE20)
Plasmid#52011PurposeExpresses the human MAVS CDS containing the point mutations M1A and M142ADepositorInsertMAVS-M1A,M142A
UseRetroviralTagsExpressionMammalianMutationchanged Met 1 to Ala and Met 142 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RNMT
Plasmid#112705PurposeExpresses N-terminally FLAG-tagged human RNMT in mammalian cellsDepositorInsertRNMT (RNMT Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-GST-RNMT
Plasmid#112709PurposeExpresses N-terminally GST-tagged human RNMT in bacterial cellsDepositorInsertRNMT (RNMT Human)
UseTagsGST and thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro-{beta}4 siRNA
Plasmid#16266DepositorInsertSIRNA Beta 4 Integrin (ITGB4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro-{beta}4 SCR
Plasmid#16267DepositorInsertSCR Beta 4 Integrin (ITGB4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RNMT 1-120
Plasmid#112706PurposeExpresses N-terminally FLAG-tagged human RNMT 1-120 in mammalian cellsDepositorInsertRNMT (RNMT Human)
UseTagsFLAGExpressionMammalianMutationdeleted amino acids 121-476PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RNMT 121-476
Plasmid#112707PurposeExpresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cellsDepositorInsertRNMT (RNMT Human)
UseTagsFLAGExpressionMammalianMutationdeleted amino acids 2-120PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAc-His-hAR-2
Plasmid#89091Purposebaculovirus transfer vector for histidine-tagged full-length human androgen receptor, starts Met-Ala-His6-Met-hARDepositorInserthAR (AR Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBRed-d2-hygro
Plasmid#139196Purposenon-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puroDepositorInsertCBRed
UseLuciferase and RetroviralTagsd2 PEST domain and nuclear localization signal (N…ExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA3-ΔN-RNMT
Plasmid#112708PurposeExpresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.DepositorInsertRNMT (RNMT Human)
UseTagsFLAG and HIV Rev nuclear export signal (NES)ExpressionMammalianMutationdeleted amino acids 2-120, mutated active-site D2…PromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
-
HA tagged Sph ELL2
Plasmid#127270PurposeFor in vitro translation of human WT ELL2 with internal HA tagDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationWT Mets and HA tag at SphI site (8th Met)PromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
-
-
-
-
HA tagged mid ELL2 M133, 138, 186ILeu
Plasmid#127276PurposeExpresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt functionDepositorInsertELL2 (ELL2 Human)
UseTagsExpressionMammalianMutationM133, 138, 186 to Ileu, HA tag at XbaI after Met …PromoterpEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
cfSYFP2-N
Plasmid#110631PurposeCys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.DepositorInsertcfSYFP2-N
UseTagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3EF5a-PIK3CA H1047R
Plasmid#180034PurposeEntry vector of PIK3CA H1047R for gateway cloning.DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseEntry vectorTagsHA tagExpressionMammalianMutationH1047RPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-PIK3CA E545K
Plasmid#180032PurposeEntry vector of PIK3CA E545K for gateway cloning.DepositorInsertPIK3CA E545K (PIK3CA Human)
UseEntry vectorTagsHA tagExpressionMammalianMutationE545KPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pJK687
Plasmid#71640PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met mutant (TdPurE2-K41M)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methioninePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH-PIK3CA H1047R
Plasmid#180031PurposeThis plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseTagsHA tagExpressionMammalianMutationH1047RPromoterEF1aAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pJK660
Plasmid#71635PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methionine, phenylalanine-79…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK650
Plasmid#72400PurposeProduces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)DepositorInsertglutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
UseTagsExpressionBacterialMutationMutated tyrosine-397 to phenylalanine to block ad…PromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHM_YEAST
Plasmid#204231PurposeBacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM_YEAST (MDH1 Budding Yeast)
UseTags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
HOXC6 (human) HIS-tag pET
Plasmid#8565DepositorInsertHOXC6 (HOXC6 Human)
UseTagsHis and T7ExpressionBacterialMutationThis protein starts at an internal Met initially …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only