We narrowed to 19,241 results for: INO

1. pAAV-Ef1a-fDIO-tdTomato (AAV1)

   Viral Prep

   #128434-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-tdTomato (#128434). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-tdTomato plasmid DNA. EF1a-driven, Flp-dependent expression of tdTomato. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Promoter

   EF1a

   Tags

   tdTomato (Flp-dependent)

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV9)

   Viral Prep

   #171027-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Promoter

   EF1a

   Tags

   tdTomato

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV1)

   Viral Prep

   #171027-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Promoter

   EF1a

   Tags

   tdTomato

   Available since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

4. pPaGE Pyl TAG FliC T248TAG

   Plasmid

   #160089

   Purpose

   Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.

   Depositor

   Lital Alfonta

   Article

   Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea

   Inserts

   Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
   Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
   Flagellin
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed Threonine 248 to TAG stop-codon.

   Promoter

   Pseudomonas aeruginosa Leu-tRNA native promoter a…

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUM

   Plasmid

   #133130

   Purpose

   A recombinant plasmid expressing fum (fumarase) gene

   Depositor

   Gregg Beckham

   Article

   Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   fumarase gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PEP-carboxykinase promoter

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPCK

   Plasmid

   #133128

   Purpose

   a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

   Depositor

   Gregg Beckham

   Article

   Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   PEP-carboxykinase gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PEP-carboxykinase promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDH

   Plasmid

   #133129

   Purpose

   A recombinant plasmid expressing mdh (malate dehydrogenase) gene

   Depositor

   Gregg Beckham

   Article

   Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   malate dehydrogenase gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   malate dehydrogenase promoter

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGU06

   Plasmid

   #212148

   Purpose

   Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.

   Depositor

   Kang Zhou

   Insert

   alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase (AGX1, alaA, tyrB Agx1: S. cerevisiae; alaA, tyrB: E. coli, Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PgyrA

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p15aC-4D1D-5

   Plasmid

   #107866

   Purpose

   Bacterial expression of PIS, PI4K and PI4P5K

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Inserts

   PIS
   phosphatidylinositol 4-phosphate 5-kinase
   phosphatidylinositol 4-kinase β
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   proD and proD (in operon)

   Available since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p15aC-3D1D-5

    Plasmid

    #121050

    Purpose

    Bacterial expression of PIS, PI4K and PI3P5K mutant E545K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K
    

    Use

    Tags

    myc and none

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAJE3-E7

    Plasmid

    #214359

    Purpose

    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

    Depositor

    Ryan Mehl

    Article

    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

    Inserts

    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AmpR and lpp

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. human rSHIP2 (18-893)

    Plasmid

    #128568

    Purpose

    Expression of human SHIP2 in bacteria

    Depositor

    Christophe Erneux

    Article

    Pesesse et al FEBS Lett. 1998 Oct 23;437(3):301-3.

    Insert

    SHIP2 (INPPL1 Human)
    

    Use

    Tags

    His Xpress (N-terminal)

    Expression

    Bacterial

    Mutation

    SHIP2 coding sequence from amino acid 18 (A) till…

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-pchD WT

    Plasmid

    #187973

    Purpose

    Expresses salicylate adenylation enzyme PchD from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Shelton et al J Biol Inorg Chem. 2022 May 5. pii: 10.1

    Insert

    pchD WT
    

    Use

    Tags

    Hexahistag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Combined γB2FL-FRET probe

    Plasmid

    #209094

    Purpose

    mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

    Depositor

    Takeharu Nagai

    Article

    Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Combined γB2ΔICD-FRET probe

    Plasmid

    #209093

    Purpose

    mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

    Depositor

    Takeharu Nagai

    Article

    Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET14b-PITPalpha

    Plasmid

    #58256

    Purpose

    bacterial expression of human PITP alpha

    Depositor

    Shamshad Cockcroft

    Article

    Hara et al J Biol Chem. 1997 Jun 6;272(23):14908-13

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pNC2

    Plasmid

    #72438

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg

    Promoter

    T7 and n/a

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSG086

    Plasmid

    #214263

    Purpose

    PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)

    Depositor

    Ute Romling

    Article

    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

    Insert

    Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    substitution of 31 amino acids of FtsH2 (F124RRFA…

    Promoter

    T7 promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBlueSKMimRNA

    Plasmid

    #102535

    Purpose

    Template for synthesis of Minos transposase mRNA

    Depositor

    Michalis Averof

    Article

    Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

    Insert

    Minos transposase mRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-RILPdel212N

    Plasmid

    #110499

    Purpose

    Dominant Negative

    Depositor

    Bernardo Mainou, Terence Dermody

    Article

    Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

    Insert

    RILPdel212N (RILP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino terminal 212 amino acids

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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