We narrowed to 13,453 results for: Mpl;

1. pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227896

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   CCDC47 homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227897

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   NCLN homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227903

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

   Plasmid

   #217665

   Purpose

   Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   OsTir1 F74G-SNAP-Blast-AAVS1
   

   Tags

   SNAP

   Expression

   Bacterial and Mammalian

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mPLD3-sgRNA-Cas9-mcherry

   Plasmid

   #199700

   Purpose

   encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry

   Depositor

   David Nemazee

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   P2A-RFP

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOC5 cloning template vector

   Plasmid

   #183424

   Purpose

   FlpOFF knock-in vector

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOC3 cloning template vector

   Plasmid

   #183422

   Purpose

   CreON FlpOFF knock-in vector

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. psiCHECK3-TMED3-Reverse-Complement

   Plasmid

   #136018

   Purpose

   The Reverse Complement of TMED3 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   TMED3 3'UTR (Reverse Complement) (TMED3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiCHECK3-ZC3H15-Reverse-Complement

   Plasmid

   #136019

   Purpose

   The Reverse Complement of ZC3H15 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   ZC3H15 3'UTR (Reverse Complement) (ZC3H15 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti EF1a GFP-mCherry-mPlin1

    Plasmid

    #189005

    Purpose

    Fluorescent reportor for lipophagy

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    GFP, mChery, Plin1 (Plin1 Mouse)
    

    Use

    Lentiviral

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE LOX Cloning template vector

    Plasmid

    #139651

    Purpose

    Cloning template for ORANGE-CAKE conditional knock-in constructs

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. hU6-pegRNA-EFS-mScarlet Template

    Plasmid

    #196037

    Purpose

    The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Insert

    mScarlet
    

    Expression

    Mammalian

    Promoter

    hU6 and EFS

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid

    #70658

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pBx1-VHH template1-3XMyc-Spacer

    Plasmid

    #176208

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template1-3XMyc-Spacer
    

    Tags

    Myc tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBx1-VHH template3-3XMyc-Spacer

    Plasmid

    #176210

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template3-3XMyc-Spacer
    

    Tags

    Myc tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBx1-VHH template2-3XMyc-Spacer

    Plasmid

    #176209

    Purpose

    PCR template for generating CeVICA VHH/nanobody library

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    VHH template2-3XMyc-Spacer
    

    Tags

    Myc tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid

    #158576

    Purpose

    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    

    Tags

    V5 and HA on the endogenous KCC2 protein product

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid

    #70659

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHnS KI;Actb Donor;3xV5 KO;Template

    Plasmid

    #240291

    Purpose

    KI:Actb Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Actb
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHnS KI;Sptbn4 Donor;3xV5 KO;Template

    Plasmid

    #240295

    Purpose

    KI:Sptbn4 Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Sptbn4
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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