We narrowed to 223 results for: YPet
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Plasmid#71711PurposeThis form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctionsDepositorInsertalpha-catenin Δβ-cat mutant FRET Sensor
TagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 48- 163PromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
VBS mutant of alpha catenin sensor
Plasmid#71710PurposeThis form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.DepositorInsertalpha-catenin ΔVBS FRET Sensor
TagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 316-405 in α -catenin…PromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr-6080NES(Epac-sensor)
Plasmid#162487PurposehyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertEpac-sensor (RAPGEF3 Human, Synthetic)
TagsYPet and Turquoise2GLExpressionMammalianPromoterCAGAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Golgi-RasAR
Plasmid#205570PurposeGolgi membrane-targeted RasAR.DepositorTagsGolgin B1 (Giantin) residues 3131-3259ExpressionMammalianPromoterCMVAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b-M3C
Plasmid#107087PurposeM. sedula PCNA3 fused at the N-terminus of CyPetDepositorInsertM. sedula PCNA3 fused to CyPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-S3C
Plasmid#107090PurposeS. solfataricus PCNA3 fused at the N-terminus of CyPetDepositorInsertS. solfataricus PCNA3 fused to CyPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-S1C
Plasmid#107088PurposeS. solfataricus PCNA1 fused at the N-terminus of CyPetDepositorInsertS. solfataricus PCNA1 fused to CyPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-M2C
Plasmid#107086PurposeM. sedula PCNA2 fused at the N-terminus of CyPetDepositorInsertM. sedula PCNA2 fused to CyPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-M1C
Plasmid#107085PurposeM. sedula PCNA1 fused at the N-terminus of CyPetDepositorInsertM. sedula PCNA1 fused to CyPet
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
JH05
Plasmid#73516PurposemYPet_PH pCFJ150 - contain C. elegans codon optimized mYPetDepositorInsertpmex-5::mPYet::PH::tbb-2
TagsmYPetPromotermex-5Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
ExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
ExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
TagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…Available SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-CFP
Plasmid#90103PurposeCFP control expression plasmidDepositorInsertCFP
UseBroad-host rangeExpressionBacterialPromoterPtacAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
LT2_15_UPRpro_X2site
Plasmid#194427PurposePlasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein ResponseDepositorInsertUPRpro
UseUsed as donor dna in genome integration after lin…ExpressionYeastMutationNAAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT2_33_PyruPro_X2site
Plasmid#194429PurposePlasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)DepositorInsertPyruPro
UseUsed as donor dna in genome integration after lin…ExpressionYeastMutationNAAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT2_7_OxPro
Plasmid#177709PurposePlasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)DepositorInsertspTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT2_14_GlyOx
Plasmid#177711PurposePlasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT2_32_PyruEth_X2site
Plasmid#194428PurposePlasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)DepositorInsertPyruEth
UseUsed as donor dna in genome integration after lin…ExpressionYeastMutationNAAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only