Addgene

We narrowed to 243 results for: cdc42

Showing: 201 - 220 of 243 results

Results per page:

pmRFP-N1 human cofilin S3A

Plasmid

#50857

Purpose

expresses constituvely active cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 human cofilin S3A

Plasmid

#50860

Purpose

expresses constituvely active cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin S3E

Plasmid

#50861

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Lck-mCherry-RGS

Plasmid

#112935

Purpose

Plasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)

Depositor

Dorus Gadella

Article

Reinhard et al Mol Biol Cell. 2017 Nov 7;28(23):3371-33

Insert

p115RhoGEF-RGS domain

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

Plasmid

#136646

Purpose

Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

Depositor

Karl Matter, Maria Balda

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1R312A (RNAi resistant) (SH3BP1 Human)

Use

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…

Promoter

Availability

Academic Institutions and Nonprofits only

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pLV_PP1-mCitrine

Plasmid

#172472

Purpose

Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

Depositor

Sean R Collins

Article

Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

Insert

prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)

Use

Lentiviral

Tags

mCitrine and prolactin signal sequence (cleaved p…

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pTriEX-Antenna-GDI Rac1

Plasmid

#83359

Purpose

Expression of Antenna-Rac1

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Rac1 (RAC1 Human)

Use

Tags

FRET Antenna (mCerulean-cpVenus) and His

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin S3E

Plasmid

#50858

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pET23-CBD

Plasmid

#30311

Depositor

Klaus Hahn

Insert

CBD (WAS Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pCMV5-FLAG-Gene33

Plasmid

#21633

Depositor

John Kyriakis

Article

Makkinje et al J Biol Chem. 2000 Jun 9. 275(23):17838-4

Insert

Gene33 (Errfi1 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
JB48

Plasmid

#128930

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Cdc42GV-BirA*-HA-MAO (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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JB57

Plasmid

#128931

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Cdc42TN-BirA*-HA-MAO (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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2xsspB-mSc-sspB-ITSN1

Plasmid

#114425

Purpose

Translocating ITSN1 to iLID and activate Cdc42

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

ITSN1

Use

Tags

mScarlet

Expression

Mammalian

Mutation

truncated ITSN1 (1159-1509)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-I-N-CAAX

Plasmid

#41686

Purpose

Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145N-IntersectinDH-Dronpa145N-CAAX

Use

Tags

CAAX box

Expression

Mammalian

Mutation

K145N on Dronpa

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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140_pSKB3-GBD-LUMP

Plasmid

#65972

Purpose

Bacterial expression of GBD-LUMP protein, a sensor for GTP-bound Cdc42

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Use

Tags

GBD (GTPase binding domain from ACK1; residues 44…

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Ypet-PBD

Plasmid

#105290

Purpose

To measure Rac or Cdc42 activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Pak1 (PAK1 Human)

Use

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 65-150

Promoter

Availability

Academic Institutions and Nonprofits only

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mMaroon-PAK-mMaroon

Plasmid

#84361

Purpose

FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mMaroon-PAK-mMaroon

Use

Tags

mMaroon flanking PAK domain on N and C terminus

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAV0756

Plasmid

#133508

Purpose

Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAV0757

Plasmid

#133509

Purpose

Expression of 3GFP tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAV0816

Plasmid

#133510

Purpose

Expression of mTagBFP2 tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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