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Plasmid#80747PurposeExpression of human beta A3-crystallin in E. coliDepositorInsertCRYBA3 (CRYBA1 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYBB3
Plasmid#80750PurposeExpression of human beta B3-crystallin in E. coliDepositorInsertCRYBB3 (CRYBB3 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYGS
Plasmid#80753PurposeExpression of human gamma S-crystallin in E. coliDepositorInsertCRYGS (CRYGS Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFU-cMVIIA-PE
Plasmid#24147DepositorInsertloxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
PB-PE VQR
Plasmid#162797PurposeAll-in-one prime editor piggyBac transposon, VQR variantDepositorInsertSpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationD1135V, R1335Q, T1337RPromoterCAGAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-PE NG
Plasmid#174554PurposeAll-in-one prime editor piggyBac transposon, NG variantDepositorInsertSpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationL1111R, G1218R, E1219F, A1322R, R1335V, T1337RPromoterCAGAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYBA1
Plasmid#80745PurposeExpression of human beta A1-crystallin in E. coliDepositorInsertCRYBA1 (CRYBA1 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFU-iMVIIA-PE
Plasmid#24146DepositorInsertomega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP-IRES-TetR-KRAB (PDGFRB Human, Conus magus, Aequorea victoria)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYBA2
Plasmid#80746PurposeExpression of human beta A2-crystallin in E. coliDepositorInsertCRYBA2 (CRYBA2 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYGB
Plasmid#80751PurposeExpression of human gamma B-crystallin in E. coliDepositorInsertCRYGB (CRYGB Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYGC
Plasmid#80754PurposeExpression of human gamma C-crystallin in E. coliDepositorInsertCRYGC (CRYGC Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYGA
Plasmid#217672PurposeFor expression of SUMO tagged human gamma A crystallinDepositorInsertCRYGA (CRYGA Human)
UseTags6XHis and Smt3ExpressionBacterialMutationPromoterT7Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYBA4
Plasmid#217671PurposeFor expression of SUMO tagged human beta A4 crystallinDepositorInsertCRYBA4 (CRYBA4 Human)
UseTags6XHis and Smt3ExpressionBacterialMutationPromoterT7Available sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pE-N1-RDH11
Plasmid#161916PurposeExpresses untagged human RDH11. Confers G418 resistance.DepositorInsertRetinol dehydrogenase 11 (RDH11 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST
Plasmid#221118PurposeFAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3-deltaPEST
UseTagsExpressionYeastMutationSTE3 mutant that lacks the PEST domain in the C-t…PromoterSTE3Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA
Plasmid#174030PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-Venus-PEST
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST
Plasmid#127332PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRESDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
Plasmid#217513PurposeEncodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD RecombinaseDepositorInsert10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
Plasmid#217515PurposeEncodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD RecombinaseDepositorInsert13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP
Plasmid#176531PurposePiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.DepositorInsertsKGV transcription factor
TagBFP
UsePiggybac transposase integration vectorTagsErkKTR, Gal4 DNA binding domain, PEST destabiliza…ExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only