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Plasmid#133497PurposeExpression of nucleo-cytoplasmic mTagBFP2 in M-cellsDepositorInsertpAde6(PmeI)-p(mam1*)-mTagBFP2-terminator(ScADH1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry-CD8-vhhGFP
Plasmid#163930PurposeExpression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pONSY-mCherry
Plasmid#111874PurposeThis plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.DepositorInsertmCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONSY-Lifeact:mCherry
Plasmid#111876PurposeThis plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.DepositorInsertLifeact fused to mCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONSY-Venus
Plasmid#111875PurposeThis plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.DepositorInsertVenus
UseCapsaspora owczarzakiTagsVenusExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p181 pK7-HDAC1 (GFP)
Plasmid#11054DepositorInsertHDAC1 (Hdac1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
Spacer Switch Plasmid
Plasmid#215346PurposeFacilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.DepositorInsertOpaD spacer; Chloramphenicol Resistance Cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV0782
Plasmid#133514PurposeExpression of sfGFP tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0783
Plasmid#133515PurposeExpression of mCherry tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0612
Plasmid#133505PurposeExpression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitCDepositorInsertpAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mHMGB4
Plasmid#31620DepositorInsertHMGB4 (Hmgb4 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAV0816
Plasmid#133510PurposeExpression of mTagBFP2 tagged CRIB domain to monitor active Cdc42DepositorInsertpAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTL
Plasmid#203176PurposeCentromeric yeast expression vector, leucine selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP
Pooled Library#153101PurposeCRISPR library targets all non-essential and conditionally essential transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP-Grp1
Pooled Library#153102PurposeCRISPR library targets a sub-pool of transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP-Grp2
Pooled Library#153103PurposeCRISPR library targets a sub-pool of transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP-Grp3
Pooled Library#153104PurposeCRISPR library targets a sub-pool of transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP-Grp4
Pooled Library#153105PurposeCRISPR library targets a sub-pool of transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP-Grp5
Pooled Library#153106PurposeCRISPR library targets a sub-pool of transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19_18GZG
Plasmid#117228Purposeshble flanked by an endogenous GAPDH promoter and terminator systemDepositorInsertshble
UseThraustochytrid expressionTagsExpressionMutationPromoterGAPDHAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only