We narrowed to 20,614 results for: ACE

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pKDsgRNA-trmI

Plasmid

#89955

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

trmI gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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BC-A1-009

Plasmid

#78696

Purpose

A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSY075

Plasmid

#110067

Purpose

Bacterial expression of L2

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L2

Expression

Bacterial

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEX Tom20 mVenus BoPL

Plasmid

#114467

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoPL

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoPL

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx TOM20 mVenus BoLL

Plasmid

#114465

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoLL

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoLL

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEX Tom20 mVenus BoLD

Plasmid

#114468

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoLD

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoLD

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-xylA

Plasmid

#89964

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

xylA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-lpoB

Plasmid

#89959

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

lpoB gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p)

Plasmid

#70197

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)

Plasmid

#70213

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

lacI

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pTU3-B-RFP

Plasmid

#72946

Purpose

Level 3 Destination vector - Assembly of 4 Level 2 modules - ABCD

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 3 - B site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 Y91F

Plasmid

#47093

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y91F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y91F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 FL H87Y C214S

Plasmid

#47087

Depositor

Marina Ramirez-Alvarado

Article

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL H87Y C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

H87Y C214S

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pNB-Rep

Plasmid

#238996

Purpose

Node B carrying the sfGPF reporter that can be used for the assembly of pEND-11Y, pEND-Y11, pEND-Z11 or pEND-ZY

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sfGFP-MarAn10 downstream of a strong constitutive promoter and a binding site for sgRNA-1

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDR-pBAD-ATG-6xHIS-AsiA

Plasmid

#245835

Purpose

Express anti-sigma protein, AsiA, in E. coli. His-Tag for Western Blots or Purification.

Depositor

Jie Xiao

Article

Weng et al Proc Natl Acad Sci U S A. 2019 Oct 1;116

Insert

AsiA

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pKO024.306-107

Plasmid

#242927

Purpose

Expression of a Sth1-dCas9 compatible MS2 scRNA J306 and a Spy-dCas9 compatible sgRNA J107

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

sgRNA J107

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23119

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).cpSFGFP.mRuby3

Plasmid

#244914

Purpose

cpSFGFP control with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mRuby3

Use

AAV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).cpSFGFP.mIRFP670nano3

Plasmid

#244918

Purpose

cpSFGFP control with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mIRFP670nano3

Use

AAV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCK761

Plasmid

#242916

Purpose

Constitutive expression of dCas9, αNTD-MCP, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCK948

Plasmid

#242917

Purpose

Constitutive expression of dCas9, MCP-TetD, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKO025

Plasmid

#242913

Purpose

aTc-inducible expression of Spy-dCas9-AsiA and Sth1-dCas9, and constitutive expression of MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

Sth1-dCas9

Use

CRISPR and Synthetic Biology

Promoter

Ptet

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCK660

Plasmid

#242914

Purpose

Constitutive expression of dCas9, MCP-SoxS, and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK071

Plasmid

#242907

Purpose

Constitutive expression of dCas9 and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK320

Plasmid

#242906

Purpose

aTc-inducible expression of dCas9-AsiA

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

AsiA (asiA Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

TetR-Ptet

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCK321

Plasmid

#242912

Purpose

aTc-inducible expression of dCas9-AsiA and constitutive expression of MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

MCP-SoxS

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-Nb19-P4S

Plasmid

#236500

Purpose

Expresses anti-Apolipoprotein A1 nanobody Nb19 as a fusion protein with coiled coil P4S in bacteria.

Depositor

Alexander Green

Article

Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

Insert

Nb19-P4S

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-202-P1

Plasmid

#236497

Purpose

Expresses anti-RiVax nanobody 202 as a fusion protein with coiled coil P1 in bacteria.

Depositor

Alexander Green

Article

Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

Insert

202-P1

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-301-P3S

Plasmid

#236498

Purpose

Expresses anti-RiVax nanobody 301 as a fusion protein with coiled coil P3S in bacteria.

Depositor

Alexander Green

Article

Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

Insert

301-P3S

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-Nb11-P2

Plasmid

#236499

Purpose

Expresses anti-Apolipoprotein A1 nanobody Nb11 as a fusion protein with coiled coil P2 in bacteria.

Depositor

Alexander Green

Article

Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

Insert

Nb11-P2

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pNS1D-sgRNA9-SpR

Plasmid

#191092

Purpose

aTc inducible gRNA expression plasmid with BbsI sites for Golden Gate insertion of crRNA oligos and homology arms for genome integration at NS1D in Synechococcus sp. PCC 7002

Depositor

Anne Ruffing

Article

Dallo et al ACS Synth Biol. 2023 Apr 21;12(4):1175-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

tet

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pProUSER13F4C

Plasmid

#178023

Purpose

E. coli - B. subtilis nicking vector pProUSER13F4C. Ap-BBR1; ΔsigF-insertion-site; Km; manR-PmanA

Depositor

Sheila Jensen

Article

Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

Insert

nicking site to be replaced with your gene of interest

Expression

Bacterial

Promoter

Pman

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pProUSER13E3F

Plasmid

#178026

Purpose

E. coli - B. subtilis nicking vector pProUSER13E3F. Ap-BBR1; yhgE-insertion-site; Spc; tetR-Ptet

Depositor

Sheila Jensen

Article

Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

Insert

nicking site to be replaced with your gene of interest

Expression

Bacterial

Promoter

Ptet

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pProUSER13G2F

Plasmid

#178027

Purpose

E. coli - B. subtilis nicking vector pProUSER13G2F . Ap-BBR1; rsbP-insertion-site; Ery; tetR-Ptet

Depositor

Sheila Jensen

Article

Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

Insert

nicking site to be replaced with your gene of interest

Expression

Bacterial

Promoter

Ptet

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb827

Plasmid

#217500

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb837

Plasmid

#217501

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb847

Plasmid

#217502

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb867

Plasmid

#217503

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb887

Plasmid

#217504

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb897

Plasmid

#217505

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pILFPQ13A7

Plasmid

#223837

Purpose

Expressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mScarletI3>

Expression

Yeast

Promoter

TEF1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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