We narrowed to 22,018 results for: ESC

1. pTfR-PAmCherry1

   Plasmid

   #31948

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Use

   Tags

   transferrin receptor (TfR)

   Expression

   Mammalian

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   CMV

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTJV1Sc3-rpoB

   Plasmid

   #166983

   Purpose

   Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Insert

   RecT (recT E. coli)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pVvanCC

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Plasmid L5 attB:Psmyc double green

   Plasmid

   #169412

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mWasabi
   mWasabi CO
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Plasmid L5 attB:Psmyc triple red

   Plasmid

   #169413

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mScarlet proper
   COmScarlet
   mRuby3
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-miRFP718nano

   Plasmid

   #195744

   Purpose

   Monomeric near-infrared fluorescent protein miRFP718nano in mammalian plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   miRFP718nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP

   Plasmid

   #200277

   Purpose

   Characterisation of the sialic acid-responsive circuit with fluorescent reporter

   Depositor

   Matthew Chang

   Article

   Koh et al Nat Commun. 2022 Jul 4;13(1):3834. doi:

   Inserts

   CadC
   NanR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AsdCas12a KRAB

   Plasmid

   #188502

   Purpose

   Expresses FLAG tagged AsdCas12a KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD/His-miRFP670nano

   Plasmid

   #127427

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670nano in bacterial plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   araC

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKeratin-Electra1

    Plasmid

    #179480

    Purpose

    Keratin labeling with blue fluorescent protein Electra1

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Keratin-Electra1
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pActin-miRFP670nano

    Plasmid

    #127428

    Purpose

    Human beta-actin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano-human beta-actin
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. PM-RA

    Plasmid

    #209878

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #126218

    Purpose

    Global labeling of excitatory synapses (green fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95.FingR-eGFP-CCR5TC
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIa

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-GINKO1

    Plasmid

    #113111

    Purpose

    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Use

    Tags

    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. LbdCas12a KRAB

    Plasmid

    #188503

    Purpose

    Expresses FLAG tagged LbdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEmCherry2

    Plasmid

    #118413

    Purpose

    Red fluorescent reporter for assessing CRISPR gRNA activity

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMyosin-miRFP670nano3

    Plasmid

    #184668

    Purpose

    LC-Myosin labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3-LC-Myosin
    

    Use

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLD1 translation factors

    Plasmid

    #117760

    Purpose

    BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 1-13
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD/HisB-Fast-FT

    Plasmid

    #31916

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Fast-FT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

    Promoter

    araB

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH2B-Electra1

    Plasmid

    #179478

    Purpose

    Nuclear labeling with blue fluorescent protein Electra1 fused to H2B

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    H2B-Electra1
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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