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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,275 results for: 110

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Showing: 2201 - 2220 of 12275 results

  4. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

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  5. pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49227
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49227-plasmid-map-sequence-id-73643

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  11. Is3xP3-mCherry

    Plasmid
    #220091
    Purpose
    Expresses in ISE6 (Ixodes scapularis) cells, heat inducible. Promoter consists of 3 Pax 6 sites from 3xP3 with a minimal IsHSP70 promoter. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega)
    Depositor
    Monika Gulia-Nuss
    Article
    Pham et al iScience. 2024 Jul 8;27(8):110468. doi:
    Insert
    3 Pax6 binding sites from 3xP3 with minimal HSP70 promoter from Ixodes scapularis
    Promoter
    Is3xP3
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    220091-plasmid-map-sequence-id-457682

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Showing: 2201 - 2220 of 12275 results