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We narrowed to 7,585 results for: pcDNA3.1

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Showing: 2201 - 2220 of 7585 results

  3. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available Since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    112060-plasmid-map-sequence-id-216934

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  10. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid
    #128746
    Purpose
    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.
    Depositor
    David Bartel
    Article
    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do
    Insert
    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    Expression
    Mammalian
    Mutation
    5 point mutations disrupting the miR-7 site
    Promoter
    CMV
    Available Since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128746-plasmid-map-sequence-id-254125

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  19. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid
    #50486
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    GFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the GFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2201 - 2220 of 7585 results