Skip to main content
Addgene
Showing: 2201 - 2220 of 31168 results

  1. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

    Plasmid
    #193769
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1ΔR198A/R210A (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193769-plasmid-map-sequence-id-380211

    Enlarge

  2. pCW-ND-Cdt1Δ499-546-HA-Puro

    Plasmid
    #193768
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1Δ499-546 (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193768-plasmid-map-sequence-id-380214

    Enlarge

  9. pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro

    Plasmid
    #169665
    Purpose
    Tier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV / PPGK / RPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169665-plasmid-map-sequence-id-365640

    Enlarge

  17. pLKO-puro-mAK1-shRNA-1

    Plasmid
    #185364
    Purpose
    For mammalian expression of shRNA: ATCTTGACTCCCTGAAGTAAC that targets mouse AK1 (adenylate kinase)
    Depositor
    Ayaz Najafov
    Insert
    AK1 (Ak1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185364-plasmid-map-sequence-id-363607

    Enlarge

  19. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid
    #171803
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-gB-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171803-plasmid-map-sequence-id-341955

    Enlarge

Showing: 2201 - 2220 of 31168 results