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pCAG-CAPN7-OSF

Plasmid

#193041

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CAPN7 L61D (1-165)

Plasmid

#193033

Purpose

Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L61D, Residues 1-165

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC-His+Strep-Opa1 (SB254)

Plasmid

#227603

Purpose

Inducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Inserts

SLC25A46 (SLC25A46 Human)
Opa1

Tags

10xHis and Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46[R247A,R347A]-His (SB257)

Plasmid

#227606

Purpose

Inducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Mutation

R247A,R347A

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)

Plasmid

#227620

Purpose

Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

R257A,R347A

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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LBR(R583Q)-FKBP-GFP

Plasmid

#222423

Purpose

To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(R583Q) (LBR Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

R583Q

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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LBR(N547D)-FKBP-GFP

Plasmid

#222422

Purpose

To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(N547D) (LBR Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

N547D

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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GPR6-DuET

Plasmid

#213286

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GPR6 (GPR6 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 myc-Rab10-mito

Plasmid

#208367

Purpose

Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10-mito (RAB10 Human)

Expression

Mammalian

Mutation

wildtype

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b+His SUMO Rab10(Q68L)

Plasmid

#207342

Purpose

Bacterial expression of His SUMO Rab10(Q68L)

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

Rab10 (RAB10 Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Q68L

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO2-RC_B

Plasmid

#145981

Purpose

Insect Expression of DmAGO2-RC

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

DmAGO2-RC (AGO2 Fly)

Expression

Insect

Mutation

A deletion of AA 51-56 and an insertion of 23 AA …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Opto-GPR6

Plasmid

#106002

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR6; Opto-GPR6; A4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR6 (GPR6 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter (-210-0)

Plasmid

#89826

Purpose

Fascin promoter for luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Promoter

SV40

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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FPGT-TNNI3K gRNA (BRDN0001145543)

Plasmid

#75902

Purpose

3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FPGT-TNNI3K gRNA (BRDN0001147862)

Plasmid

#75903

Purpose

3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FPGT-TNNI3K gRNA (BRDN0001147836)

Plasmid

#75904

Purpose

3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FPGT-TNNI3K gRNA (BRDN0001148036)

Plasmid

#75905

Purpose

3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A3 WT

Plasmid

#195849

Purpose

Expresses wild-type Homo sapiens ATP13A3

Depositor

Veerle Baekelandt

Article

Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

Insert

ATPase 13A3 (ATP13A3 Human)

Use

Lentiviral

Mutation

D498N, L956P, M850I, V855M, R858H, L675V

Promoter

CMV

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

Plasmid

#119816

Purpose

3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

Depositor

Ethan Abel

Article

Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

Inserts

Luciferase
Puromycin

Use

Lentiviral and Luciferase

Tags

EGFP and V5

Expression

Mammalian

Mutation

Stop codon removed in the frame of the V5 frame v…

Promoter

CMV/TO and Human Phosphoglycerate kinase

Available Since

Jan. 2, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)GαsL-260-NLuc

Plasmid

#207158

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAS (GNAS Human)

Expression

Mammalian

Mutation

NLuc inserted at position D(260)N

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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