We narrowed to 22,471 results for: ESC

1. mito-mGarnet

   Plasmid

   #104309

   Purpose

   codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

   Depositor

   Ulrich Nienhaus

   Article

   Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

   Insert

   mito-mGarnet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CyTERM-mGFP

   Plasmid

   #62237

   Purpose

   ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.

   Depositor

   Erik Snapp

   Article

   Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1

   Insert

   CyTERM-mGFP
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   signal anchor of rabbit cytochrome p450 and monom…

   Promoter

   CMV

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pH2B-Electra1

   Plasmid

   #179478

   Purpose

   Nuclear labeling with blue fluorescent protein Electra1 fused to H2B

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   H2B-Electra1
   

   Use

   Tags

   Electra1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Nano-lantern/pRSETB

   Plasmid

   #51969

   Purpose

   bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mKOkappa-2A-mTurquoise2

   Plasmid

   #98837

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mKOkappa-2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBAD/HisB-Fast-FT

   Plasmid

   #31916

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Fast-FT
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

   Promoter

   araB

   Available since

   Sept. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PM-RA

   Plasmid

   #209878

   Purpose

   Dimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddRFP A
   

   Use

   Tags

   Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LbdCas12a KRAB

   Plasmid

   #188503

   Purpose

   Expresses FLAG tagged LbdCas12a KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMAL-EFSep

   Plasmid

   #34627

   Depositor

   Dieter Söll, Jesse Rinehart

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Insert

   EF-Sep (tufB E. coli)
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Promoter

   Available since

   March 1, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pTJV1Sc3-rpoB

    Plasmid

    #166983

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    RecT (recT E. coli)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVvanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-Syn-VARNAM

    Plasmid

    #115554

    Purpose

    Red fluorescent voltage indicator for neuronal expression

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

    Insert

    VARNAM
    

    Use

    AAV

    Tags

    Golgi and ER export signal

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD-hfYFP

    Plasmid

    #186522

    Purpose

    Hyperfolder YFP fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    araB

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKeratin-Electra1

    Plasmid

    #179480

    Purpose

    Keratin labeling with blue fluorescent protein Electra1

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Keratin-Electra1
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSW002-PpsbA_mTurquoise2

    Plasmid

    #108237

    Purpose

    Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    PsbA promoter - mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046852

    Plasmid

    #145584

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pRSETA-qmTQ2-citrate

    Plasmid

    #225251

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-citrate
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTubulin-miRFP670nano

    Plasmid

    #127429

    Purpose

    Human alpha-tubulin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano-human alpha-tubulin
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLifeAct-miRFP713

    Plasmid

    #136562

    Purpose

    F-actin labeling with a near-infrared fluorescent protein miRFP713

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    LifeAct-miRFP713
    

    Use

    Tags

    miRFP713

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKB817 (pBra-Hfq)

    Plasmid

    #174661

    Purpose

    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

    Depositor

    Katherine Berry, Ann Hochschild

    Article

    Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

    Insert

    Hfq
    

    Use

    Tags

    alpha NTD

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPaxillin-Electra2

    Plasmid

    #184926

    Purpose

    Expresses paxillin fused to blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    paxillin-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge