We narrowed to 13,401 results for: App

1. pEN41A-T38S38

   Plasmid

   #49524

   Purpose

   Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   constitutive TetR38
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Ptb38

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN12A-P750

   Plasmid

   #49525

   Purpose

   Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   P750
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   P750

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMX-mTAF12

   Plasmid

   #44342

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TAF12 (Taf12 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3-Flag-hDcp2

   Plasmid

   #72213

   Purpose

   mammalian expression of Flag-tagged human Dcp2

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Q170R (please see depositor's comment below)

   Promoter

   CMV

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. 8xCTS1_BpiI-cloning-site_SV40polyA

   Plasmid

   #124547

   Purpose

   Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet 3d alpha tentacle delete CP

   Plasmid

   #13299

   Depositor

   John Cooper

   Article

   Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

   Insert

   capping protein alpha 1 (deletion 28 amino acids) beta 1 subunits (CAPZB Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

7. p415 Glo3 R59K Δ2x+ve

   Plasmid

   #112655

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 R59K Δ2x+ve
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R59K, mutations (R223E, K224E, K225E, K233E, K234…

   Promoter

   Met25

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p415 GFP-Glo3 Δ2x+ve

   Plasmid

   #112662

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   GFP- Glo3 Δ2x+ve
   

   Use

   Tags

   sfGFP

   Expression

   Yeast

   Mutation

   Mutations (R223E, K224E, K225E, K233E, K234E, K23…

   Promoter

   Met25

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-myc NES-mCE

   Plasmid

   #82463

   Purpose

   Expression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   NES mRNA capping enzyme (Rngtt HIV, Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK381

    Plasmid

    #71573

    Purpose

    Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)

    Depositor

    T. J. Kappock

    Article

    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

    Insert

    citrate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes aspartate-317 to glycine

    Promoter

    recA

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK382

    Plasmid

    #71574

    Purpose

    Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)

    Depositor

    T. J. Kappock

    Article

    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

    Insert

    citrate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes aspartate-317 to asparagine; silent mutat…

    Promoter

    recA

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK287

    Plasmid

    #71120

    Purpose

    Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coli

    Depositor

    T. J. Kappock

    Article

    Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK360

    Plasmid

    #71571

    Purpose

    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    acetyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes threonine-2 to alanine

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK385

    Plasmid

    #71572

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK347

    Plasmid

    #71833

    Purpose

    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genes

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to aspartate

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK348

    Plasmid

    #71832

    Purpose

    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genes

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to asparagine

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK193

    Plasmid

    #71834

    Purpose

    Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) region

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Phage lambda cloning vector with genomic dna inse…

    Tags

    Expression

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGS1112

    Plasmid

    #63784

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eSapphire-Strep-tag II followed by URA3
    

    Use

    E. coli cloning vector

    Tags

    Sapphire-Strep-tag II

    Expression

    Mutation

    Promoter

    none

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGMCK-0X750-luxAB-DAS+4

    Plasmid

    #49517

    Purpose

    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    P750

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGMEH-0X750-luxAB-DAS+4

    Plasmid

    #49519

    Purpose

    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomally

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    P750

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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