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pGGF_RT_FAST_Venus

Plasmid

#136980

Purpose

Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

Depositor

Klaus Theres

Insert

seed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator

Expression

Bacterial

Mutation

BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4/2H5

Plasmid

#71715

Purpose

Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP11 CS puroR

Plasmid

#46748

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

Depositor

Goedele Maertens, Gordon Peters

Publication

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIC306

Plasmid

#52257

Purpose

Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Publication

Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

Insert

Ska3 (SKA3 Human)

Use

Retroviral

Tags

EGFP, S tag, and TEV protease site

Expression

Mammalian

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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AP-p002

Plasmid

#31493

Depositor

David Wang

Publication

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

Half genome of WU polyomavirus

Expression

Bacterial

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pcDWUER

Plasmid

#37094

Depositor

David Wang

Publication

Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

Insert

WU polyomavirus early region (WUPyV_gp4, WUPyV_gp5 WU polyomavirus)

Expression

Mammalian

Promoter

CMV

Available since

July 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAV-TRE-C-prM-E-NS1

Plasmid

#175277

Purpose

AAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17D

Depositor

Wei Xu

Publication

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

C-prM-E-NS1 (POLY Yellow fever virus strain 17D)

Use

AAV

Promoter

tight TRE

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hMTP-flag

Plasmid

#138335

Purpose

Expresses MTP

Depositor

Mahmood Hussain

Publication

Rava et al J Biol Chem. 2006 Apr 21;281(16):11019-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MTTP (MTTP Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pVETL-Cmcs-Flag-Ataxin3Q25

Plasmid

#22118

Depositor

Henry Paulson

Publication

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
GAL 25Q+ProGFPp416

Plasmid

#15580

Depositor

Susan Lindquist

Publication

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
p39sheLL

Plasmid

#40627

Depositor

Simon Beddows

Publication

Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

Inserts

HPV39 L1
HPV39 L2

Expression

Mammalian

Mutation

Codon optimized for mammalian expression

Promoter

CMV and Polio IRES

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pCMV6-AN-HA_WT REV1

Plasmid

#201586

Purpose

Expresses human DNA repair protein REV1 with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DNA repair protein REV1 (REV1 Human)

Tags

HA

Expression

Mammalian

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Publication

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS415-GPD

Plasmid

#183940

Purpose

Control vector that has a GPD promoter with no insert

Depositor

Michael Downey

Publication

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Type

Empty backbone

Expression

Yeast

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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p5-39

Plasmid

#118088

Purpose

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Depositor

Bryan Dickinson

Publication

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Use

Synthetic Biology

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFB-CT10HF-LIC

Plasmid

#39191

Purpose

SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

Depositor

Nicola Burgess-Brown

Type

Empty backbone

Use

Baculovirus

Tags

TEV-His-FLAG

Promoter

polyhedrin

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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MSCV-N BKV ER

Plasmid

#37864

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

BKV ER (BKPyVgp5, BKPyVgp6 BK polyomavirus)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
miABE

Plasmid

#205412

Purpose

Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Publication

Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

Insert

pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR

Expression

Mammalian

Mutation

D61A+E85R+H369R+S387R+S457R

Promoter

hU6, CAG, CMV

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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His-GFP-Clathrin

Plasmid

#170858

Purpose

Mammalian expression of His-GFP-Clathrin.

Depositor

Justin Taraska

Publication

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Clathrin light polypeptide (Lca) (Clta Mouse)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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Chx10 3kb AP (CC#318)

Plasmid

#15205

Depositor

Connie Cepko

Publication

Rowan et al Dev Biol. 2005 May 15. 281(2):240-55.

Insert

Chx10 3kb AP reporter (Vsx2 Mouse)

Expression

Bacterial

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article