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pCRISPEY-Z3-Kan

Plasmid

#232102

Purpose

Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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YCe1760 HC_Kan_IRES2_p8b

Plasmid

#100673

Purpose

part designed to occupy position 8a of EMMA. Functional category: IRES sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

IRES2

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1762 HC_Kan_SV40p_p14

Plasmid

#100674

Purpose

part designed to occupy position 14 of EMMA. Functional category: Constitutional promoter

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

SV40 promoter

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1883 HC_Kan_p2A_p8

Plasmid

#100675

Purpose

part designed to occupy position 8a of EMMA. Functional category: p2A sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

P2A

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1899 HC_Kan_PuroR_p9

Plasmid

#100682

Purpose

part designed to occupy position 9 of EMMA. Functional category: Selection marker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

PuroR

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2026 HC_Kan_mRuby2_p9

Plasmid

#100687

Purpose

part designed to occupy position 9 of EMMA. Functional category: Fluorescent marker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

mRuby2

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2030 HC_Kan_bGHpA_p16

Plasmid

#100688

Purpose

part designed to occupy position 16 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

bGHpA

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2034 HC_Kan_mTagBFP2_p9

Plasmid

#100689

Purpose

part designed to occupy position 9 of EMMA. Functional category: Fluorescent marker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

mTAGBFP2

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2038 HC_Kan_CMVp_p18

Plasmid

#100690

Purpose

part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

CMV promoter

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2040 HC_Kan_PuroR_p21

Plasmid

#100691

Purpose

part designed to occupy position 21 of EMMA. Functional category: Selection marker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

PuroR

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2046 HC_Kan_PuroR_p15

Plasmid

#100692

Purpose

part designed to occupy position 15 of EMMA. Functional category: Selection marker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

PuroR

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2058 HC_Kan_Kozak_ATG_Palm_p6

Plasmid

#100694

Purpose

part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Kozak_ATG_Palm

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2060 HC_Kan_Linker3_p20

Plasmid

#100695

Purpose

part designed to occupy position 20 of EMMA. Functional category: Peptide linker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Linker3

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2064 HC_Kan_p2A_p20

Plasmid

#100696

Purpose

part designed to occupy position 20 of EMMA. Functional category: p2A sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

P2A

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2741 HC_Kan_EF1ap_p18

Plasmid

#100698

Purpose

part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EF1a promoter (EEF1A1 Human)

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2746 HC_Kan_Linker1_p8

Plasmid

#100699

Purpose

part designed to occupy position 8 of EMMA. Functional category: Peptide linker

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Linker1

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe3752 HC_Kan_IRES2_p20

Plasmid

#100702

Purpose

part designed to occupy position 20 of EMMA. Functional category: IRES sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

IRES2

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2982 HC_Kan_conn_B-E

Plasmid

#100644

Purpose

EMMA toolkit Assembly connector B-E

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector B-E

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2984 HC_Kan_conn_B-L

Plasmid

#100645

Purpose

EMMA toolkit Assembly connector B-L

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector B-L

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1921 HC_Kan_conn_H-K

Plasmid

#100646

Purpose

EMMA toolkit Assembly connector H-K

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EMMA toolkit Assembly connector H-K

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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