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pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

Plasmid

#169191

Purpose

To express nsp10-nsp16 fusion protein in E. coli

Depositor

John Diffley

Article

Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

Insert

14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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P530_SIX6-p2A-h2b-EGFP

Plasmid

#239101

Purpose

Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of SIX6. This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

Depositor

Karl Wahlin

Article

Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

Insert

SIX6 (SIX6 Human)

Use

Donor plasmid

Tags

p2A-h2b-EGFP

Expression

Mammalian

Promoter

Endogenous SIX6 promoter

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_NSP16-VA

Plasmid

#191215

Purpose

Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

Non structural protein 16 (ORF1ab SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

Plasmid

#207356

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pKT2-ACVR1-G328V-IRES-Katushka

Plasmid

#135002

Purpose

Expresses ACVR1 with a G328V mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Mendez et al Clin Cancer Res. 2020 Aug 1;26(15):4080-

Insert

Activin A receptor, type I (ACVR1) G328V (ACVR1 Human)

Expression

Mammalian

Mutation

c.983G>T, p.G328V

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) IGF1R

Plasmid

#107499

Purpose

Expresses IGF1R, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

IGF-1R (IGF1R Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

Plasmid

#184556

Purpose

Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

Depositor

Sandeep Prabhu

Article

Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

Insert

Kirsten rat sarcoma virus (Kras Mouse)

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMY.PRC2

Plasmid

#234582

Purpose

Expression of ProtA-EZH2, SUZ12, EED and RBBP4

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)

Expression

Yeast

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEF2-FL-hMer_gR1

Plasmid

#225993

Purpose

Expresses a chimeric contruct having N-terminal human MerTK and C-terminal human Interferon Gamma Receptor

Depositor

Raymond Birge, Sergei Kotenko

Article

Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

Insert

human MerTK N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (MERTK, IFNGR1 Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

Plasmid

#135766

Purpose

This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

Depositor

Nadia Mercader Huber

Article

Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

Insert

Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)

Use

Zebrafish

Promoter

UAS

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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