We narrowed to 21,210 results for: ato

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pFX-Venus-KRasCT

Plasmid

#175834

Purpose

Express Venus with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-Venus

Plasmid

#174187

Purpose

Express Venus with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gLacZ.hSyn.H2B.RFP

Plasmid

#170369

Purpose

Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pESUMO-hRORg2

Plasmid

#170329

Purpose

Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Article

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 2 (RORC Human)

Tags

HisSUMO

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

Plasmid

#162918

Purpose

Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

firefly luciferase/green fluorescent protein fusion with upstream IRES sequence

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pG.Lantern-SM50-GFP

Plasmid

#135593

Purpose

Expression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus

Depositor

Koji Akasaka, Mamiko Yajima

Article

Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

Insert

SM50 promoter (SM50 S. purpuratus)

Use

In vitro transcription (mrna synthesis)

Tags

Green Lantern GFP

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST

Plasmid

#175017

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

OK-HEFwtED

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLN481 (FuGW-G8p-IL12-1Pe)

Plasmid

#105191

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-IL12-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGREAT4

Plasmid

#170892

Purpose

GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pG10-90

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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