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CMV_4xmts_HaloTag

Plasmid

#251345

Purpose

Mitochondria matrix targeted HaloTag with 4 mitochondria targeting sequences. CMV promoter.

Depositor

Edward Avezov

Article

Parutto et al Nat Commun. 2026 Feb 11;17(1):2639. doi:

Insert

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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N-term APEX NF1

Plasmid

#253089

Purpose

APEX-tagged NF1 protein on N-term

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

NF1 (NF1 Human)

Tags

APEX

Expression

Mammalian

Available Since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

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pDD2270 pCS2-NES-arrb2a-(ENLYFQ/S)x3-SV40NLS-sfCherry3C(1-10)

Plasmid

#251767

Purpose

TEVp substrate (three-peat motif) for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

arrestin, beta 2a (arrb2a Zebrafish)

Tags

NES and NLS (SV40), sfCherry3C large fragment

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDD1707 pCS2-NES-arrb2a-(DYDIPTTENLYFQ/SGTVDAG)x1-SV40NLS-sfCherry3C(1-10)

Plasmid

#251768

Purpose

TEVp substrate (extended motif) for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

arrestin, beta 2a (arrb2a Zebrafish)

Tags

NES and NLS (SV40), sfCherry3C large fragment

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDD3254 pCS2-NES-arrb2a-GGSGGSG-ENLYFQ/S-GGSGGS-SV40NLS-sfCherry3C(1-10)

Plasmid

#251769

Purpose

TEVp substrate (GS-flanked motif) for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

arrestin, beta 2a (arrb2a Zebrafish)

Tags

NES and NLS (SV40), sfCherry3C large fragment

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDD0257 pCS2-sfCherry11Mx4-10aa-zH2B-SV40NLS-sfGFP-NPLnls

Plasmid

#251772

Purpose

Nuclear reporter for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

H2B (hist2h2l Zebrafish)

Tags

4x sfCherry3C small fragments, NLS (SV40) and NLS…

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgSOS1-2

Plasmid

#253093

Purpose

CRISPRi single-guide against SOS1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

SOS1 (SOS1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgNF1-1

Plasmid

#253096

Purpose

CRISPRi single-guide against NF1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

NF1 (NF1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgSOS1-1

Plasmid

#253092

Purpose

CRISPRi single-guide against SOS1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

SOS1 (SOS1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pUC-FLAG-V2-REPAIR-mini

Plasmid

#252298

Purpose

Expresses REPAIR-mini (V2) in mammalian cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

REPAIR-mini (V2)

Expression

Mammalian

Mutation

dPspCas13b H133A/H1058A/Δ929–1090; 30-aa linker; …

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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