168,004 results

1. pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (AAV8)

   Viral Prep

   #137163-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (#137163). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of ChR2-EYFP for optogenetic activation (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Cre-dependent)

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

2. lenti-sgRNA blast

   Plasmid

   #104993

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAC-lacZ

   Plasmid

   #13422

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Bacterial

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDAC627

   Plasmid

   #195240

   Purpose

   Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDV044

   Plasmid

   #191824

   Purpose

   Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR

   Depositor

   Jim Roberts

   Article

   Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TurboID-V5_pRS415

   Plasmid

   #107167

   Purpose

   expresses V5-tagged TurboID in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Yeast

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. EC64420

   Plasmid

   #209517

   Purpose

   Cloning in complete guide expression cassettes, with Cas9

   Depositor

   Cristobal Uauy, Wendy Harwood

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC25A51

   Plasmid

   #132019

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A51 (SLC25A51 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. hKDR

   Plasmid

   #83436

   Purpose

   human VEGFR2 with V5 and tGFP tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

   Insert

   human VEGFR2 (KDR Human)
   

   Tags

   V5 and turboGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-VSVG

    Plasmid

    #11912

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Expression

    Mammalian

    Mutation

    F64L, S65T, A206K relative the wild type GFP sequ…

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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