We narrowed to 19,691 results for: ACE

1. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

   Plasmid

   #193317

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4FLAG N-terminal tagging
   

   Use

   Tags

   4FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGWT35S-VC155

   Plasmid

   #194051

   Purpose

   Transient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   VC155 (mVenus β-strands 8 to 11, amino acids 155−239)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGWT35S-VN154

   Plasmid

   #194050

   Purpose

   Transient expression of VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Cytosol)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   VN154 (mVenus β-strands 1 to 7, amino acids 1−154)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GB2890

   Plasmid

   #193133

   Purpose

   A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.

   Depositor

   Diego Orzaez

   Article

   Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

   Insert

   GB_SynP (A2) G1dG2b.1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GB1839

   Plasmid

   #193103

   Purpose

   GB-cassette for the expression of guide RNA targeting the DFR promoter in -376 position (gRNA2), with 2.1 Ms2 aptamer in the 3' of the scaffold.

   Depositor

   Diego Orzaez

   Article

   Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

   Insert

   GB_SynP gRNA2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA-RCC

   Plasmid

   #190635

   Purpose

   Mammalian expression of genetically encoded calcium indicator RCC

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   RCC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCK411.RR1

   Plasmid

   #192644

   Purpose

   Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   BBa_J23119-RR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23119

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DpbdCas12e VPR

   Plasmid

   #188515

   Purpose

   Expresses FLAG tagged DpbdCas12e VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12e
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D672A/E769A/D935A

   Promoter

   EF1a

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO.1 - TRC mTagBFP2-Synapsin1a

   Plasmid

   #191567

   Purpose

   Expresses an shRNA and mTagBFP2-synapsin1a

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA-SCcpV

    Plasmid

    #190636

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCcpV

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCcpV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DpbCas12e

    Plasmid

    #188495

    Purpose

    Expresses FLAG tagged DpbCas12e

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PlmCas12e

    Plasmid

    #188496

    Purpose

    Expresses FLAG tagged PlmCas12e

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DpbdCas12e KRAB

    Plasmid

    #188505

    Purpose

    Expresses FLAG tagged DpbdCas12e KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D672A/E769A/D935A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cas12j3

    Plasmid

    #188497

    Purpose

    Expresses FLAG tagged Cas12j3

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12j3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTE1069

    Plasmid

    #186629

    Purpose

    SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

    Depositor

    Eriko Takano

    Article

    Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

    Inserts

    scbR
    scbAp promoter
    

    Use

    Synthetic Biology

    Tags

    6xArg

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23100 and scbAp

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSub-Asp6-MBP-HRas

    Plasmid

    #185758

    Purpose

    Bacterial expression of Asp6-MBP-HRas

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    Asp6-MBP-HRas
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    6x Asp added to the N terminus of MBP

    Promoter

    tac

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-PGK-SNAP-MDMUT-OCT4

    Plasmid

    #115692

    Purpose

    Constitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for control

    Depositor

    David Suter

    Article

    Friman et al eLife. 2019 Dec 3;8. pii: 50087. doi: 10

    Insert

    SNAP-MDMUT-OCT4
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NCH2exp

    Plasmid

    #131019

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

    Insert

    NCH2
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Promoter

    P35S

    Available since

    May 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. MNEAP1ec

    Plasmid

    #131020

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

    Insert

    MNEAP1
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. MNEAP1exp

    Plasmid

    #131021

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

    Insert

    MNEAP1
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Promoter

    P35S

    Available since

    May 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. MKAKU41ec

    Plasmid

    #131014

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

    Insert

    MKAKU41
    

    Use

    Gateway entry vector

    Tags

    mCherry-FLAG-HA

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MKAKU41exp

    Plasmid

    #131015

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

    Insert

    MKAKU41
    

    Use

    Tags

    mCherry-FLAG-HA

    Expression

    Plant

    Mutation

    Promoter

    P35S

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NCH1ec

    Plasmid

    #131016

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

    Insert

    NCH1
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. NCH1exp

    Plasmid

    #131017

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

    Insert

    NCH1
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Promoter

    P35S

    Available since

    May 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. NCH2ec

    Plasmid

    #131018

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

    Insert

    NCH2
    

    Use

    Gateway entry vector

    Tags

    eGFP-FLAG-HA

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. Soat2_Luc

    Plasmid

    #177623

    Purpose

    Cloned human Soat2 promoter containing exon1 and 2 coupled to luciferase

    Depositor

    Thomas Burris

    Article

    Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

    Insert

    human Soat2
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMC3-TPK2

    Plasmid

    #176180

    Purpose

    yeast MoClo level-0 part plasmid type 3 containing TPK2 gene

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    TPK2 (TPK2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMC8b-XI1up

    Plasmid

    #176166

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XI-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMC7-XI1dw

    Plasmid

    #176167

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XI-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMC8b-XI2up

    Plasmid

    #176168

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XI-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMC7-XI2dw

    Plasmid

    #176169

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XI-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMC8b-XII2up

    Plasmid

    #176174

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XII-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMC7-XII2dw

    Plasmid

    #176175

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XII-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMC8b-X4up

    Plasmid

    #176164

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-4

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site X-4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMC7-X4dw

    Plasmid

    #176165

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-4

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site X-4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEXP5-NT/6xHis mCherry F30 Broccoli

    Plasmid

    #169234

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-Broccoli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PROM5_MprbcL_short_chlo

    Plasmid

    #165161

    Purpose

    L0 part - promoter

    Depositor

    Jim Haseloff

    Article

    Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

    Insert

    Marchantia polymorpha rbcL promoter region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. 5UTR_Mprbcl_chlo

    Plasmid

    #163934

    Purpose

    L0 part - 5'UTR

    Depositor

    Jim Haseloff

    Article

    Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

    Insert

    Marchantia polymorpha rbcL 5'UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. L1-Ck2-smR-chlo

    Plasmid

    #163939

    Purpose

    L1 in position 2, for spectinomycin in chloroplast transformation

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    aadA gene for spectinomycin resistance
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. PROM_Ntpsba_chlo

    Plasmid

    #163950

    Purpose

    L0 part - promoter

    Depositor

    Jim Haseloff

    Article

    Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

    Insert

    Nicotiana tabacum psbA promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge