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TAL2055

Plasmid

#39413

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-28-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pAM102

Plasmid

#226694

Purpose

6xHis-Sumo-Ub-Ub-Ub-Ub

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

UB (UBI4 Budding Yeast)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-mScarlet-I(+5)

Plasmid

#219418

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

mScarlet-I(+5)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

D125K, D186K, E216K, E238K

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET51b_hSPR

Plasmid

#187111

Purpose

Expression of hSPR in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

hSPR

Tags

Hisx10-TEV

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDHJS4 AN-

Plasmid

#78247

Purpose

One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 AN+

Plasmid

#78246

Purpose

One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
JW790

Plasmid

#64813

Purpose

BiFC rTCP4

Depositor

Detlef Weigel

Article

Rubio-Somoza et al PLoS Genet. 2013 Mar;9(3):e1003374. doi:

Insert

TCP4 (TCP4 Mustard Weed)

Tags

N-terminal domain Firefly

Mutation

rTCP4

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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UPR pBARUra3

Plasmid

#45354

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

DHFR Ub Rek Ura3

Expression

Yeast

Promoter

pCUP1

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
UPR pBAM Ura3

Plasmid

#45355

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

DHFR Ub Mek Ura3

Expression

Yeast

Promoter

pCUP1

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
XNOR-gate-V2.0-ASV

Plasmid

#44455

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

XNOR-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pET28b-sad1-H79A

Plasmid

#90303

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Mutation

H79A

Availability

Academic Institutions and Nonprofits only

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pET28b-sad1-H85A

Plasmid

#90304

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Mutation

H85A

Availability

Academic Institutions and Nonprofits only

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pET28b-sad1-D159A

Plasmid

#90305

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Mutation

D159A

Availability

Academic Institutions and Nonprofits only

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pET28b-sad1-H405A

Plasmid

#90306

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Mutation

H405A

Availability

Academic Institutions and Nonprofits only

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pET28b-sad1-D422A

Plasmid

#90307

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Mutation

D422A

Availability

Academic Institutions and Nonprofits only

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pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

Plasmid

#172910

Purpose

Mammalian expression of voltage sensor; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Voltron2-ST

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pScaffold-H1 donor

Plasmid

#118152

Purpose

PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Type

Empty backbone

Expression

Mammalian

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-VARNAM A122D WPRE-bGH-polyA

Plasmid

#180486

Purpose

Mammalian expression of voltage sensor

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

VARNAM A122D

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_tBFP_PGK_mCherry

Plasmid

#79130

Purpose

Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

tBFP

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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