We narrowed to 4,855 results for: U6...

1. PX330_ZBTB7B_2

   Plasmid

   #135747

   Purpose

   Encodes gRNA for 3' target of human ZBTB7B

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZBTB7B gRNA (ZBTB7B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX330_ZBTB7B_1

   Plasmid

   #135746

   Purpose

   Encodes gRNA for 3' target of human ZBTB7B

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZBTB7B gRNA (ZBTB7B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-MRPsg3

   Plasmid

   #87191

   Purpose

   disruption of human RMRP gene via CRISPR-Cas9, guide 3

   Depositor

   Thomas Cech

   Article

   Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

   Insert

   human MRP RNA CRISPR guide #3 (RMRP Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR - C16orf80 sgRNA 1

   Plasmid

   #70651

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C16orf80
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

5. lentiCRISPR - C16orf80 sgRNA 2

   Plasmid

   #70650

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C16orf80
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. lentiCRISPR - C3orf17 sgRNA 2

   Plasmid

   #70653

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C3orf17
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pSicoR-ADAM21i1

   Plasmid

   #59604

   Purpose

   Expression of shRNA against human ADAM21

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   ADAM21 (ADAM21 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pSicoR-SLC17A5i1

   Plasmid

   #59598

   Purpose

   Expression of shRNA against human SLC17A5

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   SLC17A5 (SLC17A5 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSicoR-PTPRJi2

   Plasmid

   #59609

   Purpose

   Expression of shRNA against human PTPRJ

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   PTPRJ (PTPRJ Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pSicoR-SLC17A5i2

    Plasmid

    #59599

    Purpose

    Expression of shRNA against human SLC17A5

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    SLC17A5 (SLC17A5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pSicoR-UBE2D3i2

    Plasmid

    #59589

    Purpose

    Expression of shRNA against human UBE2D3

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    UBE2D3 (UBE2D3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

12. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cas9-GFP_sg_mAMPKa2

    Plasmid

    #79005

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa2 (Prkaa2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pDECKO_TFRC_C (with mCherry)

    Plasmid

    #72627

    Purpose

    Expresses two gRNAs targeting the TFRC promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward TFRC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0043-PspCas13b crRNA backbone

    Plasmid

    #103854

    Purpose

    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6-BbsI-BbsI-PspCas13b DR-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAc-sgRNA-Cas9

    Plasmid

    #49330

    Purpose

    Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. TLCV2

    Plasmid

    #87360

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-2A-eGFP
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Cas9-T2A-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. BRCA1 A10.2 gRNA

    Plasmid

    #90549

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA1 (Guide Designation A10.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX333-T2A-EGFP

    Plasmid

    #197417

    Purpose

    SpCas9-T2A-EGFP with dual sgRNA cloning backbones

    Depositor

    Yeh-Hsing Lao

    Article

    Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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