We narrowed to 165,918 results for: addgene

1. pCW-eGFP

   Plasmid

   #162823

   Purpose

   Doxycycline-inducible overexpression of eGFP

   Depositor

   Matthew Steinhauser

   Article

   Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

   Insert

   eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   V163A

   Promoter

   Tet ON

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFLAG-SURF4

   Plasmid

   #170680

   Purpose

   Expresses FLAG-tagged SURF4 in mammalian cells.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   SURF4
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKL10-mRuby-iATPSnFR1.0 bb118

   Plasmid

   #158980

   Purpose

   Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   mruby-iATPSNfR1.0
   

   Tags

   mruby3

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#102557

   Promoter

   Bba_J23118 (sigma 70)

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pL1P4 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198048

   Purpose

   MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMIII-Neo

   Plasmid

   #153422

   Purpose

   MSCV with IRES-Neomycin resistant gene

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTK229

   Plasmid

   #71256

   Purpose

   luciferase reposter with 229 bp of the HSV TK promoter

   Depositor

   Peter Cockerill

   Article

   Hawwari et al J Immunol. 2002 Aug 15;169(4):1876-86.

   Insert

   Thymidine Kinase promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   HSV Thymidine Kinase

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Crimson/P2A-ICP8 (pSLIK1)

   Plasmid

   #113856

   Purpose

   For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   ICP8
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Crimson/P2A-Control (pSLIK3)

   Plasmid

   #113858

   Purpose

   An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   E2-Crimson
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBEVY-U

   Plasmid

   #51230

   Purpose

   bi-directional expression vector for yeast, constitutively active, URA3 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   GPD/ADH1

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tTA_BFP

    Plasmid

    #58854

    Purpose

    Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    tTA transcription factor fused to enhanced blue fluorescent protein
    

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. miReporter-CAG

    Plasmid

    #82478

    Purpose

    microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiGuide-TagBFP-2A-BSD

    Plasmid

    #167929

    Purpose

    Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mTB3-BC2 nanobody

    Plasmid

    #210208

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    BC2 Nanobody
    

    Expression

    Bacterial

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pL1P3 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198047

    Purpose

    MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDIRE

    Plasmid

    #26745

    Depositor

    Rolf Zeller

    Article

    Osterwalder et al Nat Methods. 2010 Oct 17

    Insert

    iCre expression cassette
    

    Use

    Cre/Lox; Flp/frt

    Expression

    Mammalian

    Available Since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p-dCas9-LSD1-Hygro

    Plasmid

    #104406

    Purpose

    transient expression of dCas9-LSD1 fusion protein

    Depositor

    Stephan Beck

    Insert

    LSD1 (KDM1A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    IRATp970A0364D (Source Bioscience) has a P405H ch…

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p8266 LentiCRISPR v2 hygro sgNT-1

    Plasmid

    #193977

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. miniCMV-iRFP670-24xMS2

    Plasmid

    #238915

    Purpose

    Contains miniCMV promoter expressing iRFP670 mRNA taged 24xMS2 motif

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

    Insert

    iRFP670-24xMS2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LacZ_pHIVEGFP

    Plasmid

    #191251

    Purpose

    Expresses LacZ in mammalian cells (used as a control)

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

    Insert

    LacZ
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-puro-shFAM134B

    Plasmid

    #109013

    Purpose

    short-hairpin knockdown of targeted gene

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    shFAM134B (RETREG1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge