We narrowed to 3,140 results for: cat.3

1. px330 Gatad2a sgRNA (for deletion)

   Plasmid

   #110814

   Purpose

   Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

   Depositor

   Jacob Hanna

   Article

   Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

   Insert

   mGataD2a (Gatad2a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-CDX2-WT

   Plasmid

   #16553

   Depositor

   Bert Vogelstein

   Article

   da Costa et al Oncogene. 1999 Sep 2. 18(35):5010-4.

   Insert

   CDX2 (CDX2 Human)
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

3. SUMO_TwinStrep_Csx30

   Plasmid

   #197611

   Purpose

   For protein purification of Csx30

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   SUMO_TwinStrep_Csx30
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)

   Viral Prep

   #112677-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

5. FLAG-OTUB1 WT-pcDNA3.1

   Plasmid

   #118209

   Purpose

   Expresses FLAG-OTUB1 WT in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-Jmjd3-F

   Plasmid

   #17440

   Purpose

   Expression of Jmjd3 in mammalian cells

   Depositor

   Kai Ge

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

   Insert

   JMJD3 (Kdm6b Mouse)
   

   Use

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCOLA banana sensor sfGFP

   Plasmid

   #107367

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   banana-detecting toehold + sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCOLA kiwi sensor sfGFP

   Plasmid

   #107368

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   kiwi-detecting toehold + sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-hPDI

   Plasmid

   #60555

   Purpose

   enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

   Depositor

   Han Choe

   Article

   Song et al Appl Biochem Biotechnol. 2013 May;170(1)

   Type

   Empty backbone

   Use

   Tags

   His6-hPDI

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-CDX2-MUT

    Plasmid

    #16554

    Depositor

    Bert Vogelstein

    Article

    da Costa et al Oncogene. 1999 Sep 2. 18(35):5010-4.

    Insert

    CDX2 (CDX2 Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    RKO mutation

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pDEST-hPDIb′a′

    Plasmid

    #60556

    Purpose

    enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

    Depositor

    Han Choe

    Article

    Song et al Appl Biochem Biotechnol. 2013 May;170(1)

    Type

    Empty backbone

    Use

    Tags

    His6-hPDIb'a' tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-AaCas12b(R911A)-2AeGFP

    Plasmid

    #121961

    Purpose

    Mammalian expression, Transcription regulation, deactivated AaCas12b

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    Catalytically deactivated AaCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    R911A

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-FRB(PLF)-HA no-pA

    Plasmid

    #82597

    Purpose

    3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRB-HA (no pA)
    

    Use

    Tags

    Expression

    Mutation

    K2095P, T2098L, W2101F

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-AaCas12b(D977A)-2AeGFP

    Plasmid

    #121962

    Purpose

    Mammalian expression, Transcription regulation, deactivated AaCas12b

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    Catalytically deactivated AaCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    D977A

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-U6-AasgRNA3.8.3

    Plasmid

    #121959

    Purpose

    Mammalian expression, Transcription regulation, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA, MS2 hairpin inserted
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL0_DE-3xStop

    Plasmid

    #202151

    Purpose

    Level 0 plasmid containing 3 consecutive stop codons with DE overhangs used to build a level 1 constuct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    3x Stop codon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGTy1mhis3-AI or pGTy1his3-AI

    Plasmid

    #62228

    Purpose

    A Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.

    Depositor

    David Garfinkel

    Article

    Curcio et al Proc Natl Acad Sci U S A. 1991 Feb 1;88(

    Insert

    Ty1H3 retrotransposon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Feb. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. p201N 5770

    Plasmid

    #55772

    Purpose

    Contains soybean miRNA miR5770 recognition sequence (TCTTGTCCAAACCATAGTCCAA) to produce siRNAs from 3' target sequences and induce RNA silencing

    Depositor

    Wayne Parrott

    Article

    Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    GmUbi

    Available since

    Sept. 26, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. Rictor_2 shRNA

    Plasmid

    #1854

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-RPL3-Δ1~34,Δ215~284-TwStrep

    Plasmid

    #208630

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    Δ1~34,Δ215~284

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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