We narrowed to 13,608 results for: crispr cas9

1. PET-B-HypaR-SpCas9-NLS-6xHis

   Plasmid

   #207388

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaR-SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R661A, N692A, M694A, Q695A, H698A, amino acids 10…

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PET-B-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207378

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

   Plasmid

   #176236

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176237

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176238

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

   Plasmid

   #133791

   Purpose

   U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   St1Cas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   V1 sgRNA architecture from Carter et al. biorxiv …

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

   Plasmid

   #133792

   Purpose

   U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   St3Cas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   sgRNA architecture from Muller et al. Molecular T…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. iC30 PB-Zim3-dCas9-P2A-BFP_hygro

   Plasmid

   #236743

   Purpose

   Constitutive expression of Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
   EF1a-hygro-WPRE
   

   Expression

   Mammalian

   Promoter

   CAG and EF1a

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dCas9 plasmid

   Plasmid

   #100091

   Purpose

   dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFlag-NLS-dCas9-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SaCas9 TadCBEd

    Plasmid

    #193845

    Purpose

    Express TadCBEd (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd with SaCas9
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SauKKHCas9 PE2*

    Plasmid

    #169852

    Purpose

    Mammalian Expression, sauCas9-KKH based prime editor

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SaCas9KKH-N580A-M-MLV
    

    Tags

    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SpCas9 TadCBEa

    Plasmid

    #193782

    Purpose

    Express TadCBEa (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa-SpCas9D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SpCas9-NRTH

    Plasmid

    #198541

    Purpose

    Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRTH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAVS1_Cas9-hGem_gRNA2

    Plasmid

    #217659

    Purpose

    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA2 targeting the human AAVS1 safe harbour locus
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SaCas9 TadCBEa

    Plasmid

    #193844

    Purpose

    Express TadCBEa (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    SaCas9 TadCBEa
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SpyCas9-AIDx

    Plasmid

    #135343

    Purpose

    Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpCas9-NRRH

    Plasmid

    #198540

    Purpose

    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRRH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SpCas9-NRCH

    Plasmid

    #198542

    Purpose

    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-Cas9-NS-A

    Plasmid

    #207823

    Purpose

    Dual expression of Cas9 and non-specific sgRNA

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    non-specific sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Promoter

    EF-1a; U6

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-Cas9-NS-B

    Plasmid

    #207824

    Purpose

    Dual expression of Cas9 and non-specific sgRNA

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    non-specific sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Promoter

    EF-1a; U6

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge