We narrowed to 3,871 results for: mak

1. pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

   Plasmid

   #208872

   Purpose

   For expression in E. coli of NAP1 (delta-NDP52)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   S37K/A44E (disrupts NDP52 binding)

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

   Plasmid

   #208873

   Purpose

   For expression in E. coli of NAP1 (delta-TBK1)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   L226Q/L233Q (disrupts TBK1 binding)

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)

   Plasmid

   #208863

   Purpose

   Stably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   S37K/A44E (disrupts NDP52 binding)

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)

   Plasmid

   #208864

   Purpose

   Stably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   I11S/L12S (disrupts FIP200 binding)

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)

   Plasmid

   #208865

   Purpose

   Stably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   L226Q/L233Q (disrupts TBK1 binding)

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. YIp128-CUP1-His6-CUbo(K48R)-GFP

   Plasmid

   #212793

   Purpose

   Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   His6-CUbo(K48R)-GFP
   

   Use

   Integrative vector

   Expression

   Yeast

   Mutation

   CUb(K48R)

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIp128-CUP1-His6-CUbo(K63R)-GFP

   Plasmid

   #212794

   Purpose

   Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   His6-CUbo(K63R)-GFP
   

   Use

   Integrative vector

   Expression

   Yeast

   Mutation

   CUb(K63R)

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIp211-ADH-NUbo-E3(48) aa24-150-VSV

   Plasmid

   #212747

   Purpose

   Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(48) aa24-150
   

   Use

   Integrative vector

   Tags

   NUbo and VSV

   Expression

   Yeast

   Mutation

   Cue1 ΔU7BD

   Promoter

   ADH1

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

   Plasmid

   #212738

   Purpose

   Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Integrative vector

   Tags

   CUbo-NLS-VSV and myc

   Expression

   Yeast

   Mutation

   HOIP(C885A)

   Promoter

   ADH1

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

    Plasmid

    #212805

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
    

    Expression

    Mammalian

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. phROSA26-Tet-HA-JLP_V55Q

    Plasmid

    #208771

    Purpose

    Tetracycline inducible expression vector for HA-JLP_V55Q mutant protein, used as a donor plasmid for HA-JLP_V55Q knock-in at the human ROSA26 locus

    Depositor

    Katsuji Yoshioka

    Article

    Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

    Insert

    JLP (SPAG9 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LARP1_323-509

    Plasmid

    #209800

    Purpose

    Bacterial expression of human LARP1 La-module

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 La-module (LARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-509 only

    Promoter

    T7

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LARP1_323-410

    Plasmid

    #209801

    Purpose

    Bacterial expression of human LARP1 LaM domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 LaM (LARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-410 only

    Promoter

    T7

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4-Xpress-GBF1(E794K)

    Plasmid

    #206418

    Purpose

    Mammalian expression of hGBF1(E794K)

    Depositor

    Kazuhisa Nakayama

    Article

    Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

    Insert

    GBF1 (GBF1 Human)
    

    Expression

    Mammalian

    Mutation

    E794K

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBMN HA-ATG13(Δ1-198)

    Plasmid

    #186222

    Purpose

    For expression of ATG13(Δ1-198) tagged with HA

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1 - 198

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

    Plasmid

    #198037

    Purpose

    Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    GST-TEV-EGFP

    Expression

    Insect

    Mutation

    E696K

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac-GST-TEV-mCherry-TBK1

    Plasmid

    #198033

    Purpose

    Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    GST-TEV and mCherry

    Expression

    Insect

    Mutation

    E696K

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330A-Yap1-E1-#2

    Plasmid

    #171519

    Purpose

    deletion of a genomic locus in Yap1 gene

    Depositor

    Hans Schöler

    Article

    Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

    Insert

    Yap1 (Yap1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVPSBA01-SARS-CoV2-nsp1

    Plasmid

    #192171

    Purpose

    express SARS2-NSP1

    Depositor

    Sidi Chen

    Article

    Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

    Insert

    express SARS2-NSP1 (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVPSBA01-SARS-CoV2-nsp1 mutant4

    Plasmid

    #192174

    Purpose

    express SARS2-NSP1-mut4

    Depositor

    Sidi Chen

    Article

    Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

    Insert

    express SARS2-NSP1-mut4 (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge