We narrowed to 17,226 results for: emb

1. pCS2+-Y276H PHYB-CAAX

   Plasmid

   #154911

   Purpose

   1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.

   Depositor

   Jonathan Clarke, Clare Buckley

   Article

   Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

   Insert

   Phytochrome B (PHYB Mustard Weed)
   

   Use

   Xenopus/avian/zebrafish

   Tags

   CAAX membrane moiety and mCherry

   Expression

   Mammalian

   Mutation

   1-621 amino acids of Arabidopsis PHYB, Tyrosine r…

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FB013

   Plasmid

   #119710

   Purpose

   TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKI-cjACTB-EGFP-5'3'short

   Plasmid

   #129750

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   ACTB (ACTB C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSOX17-T2A-dTom-PGK-Neo

   Plasmid

   #223190

   Purpose

   Tomato reporter plasmid targeting the human SOX17 gene

   Depositor

   Matthew Birket

   Article

   Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

   Insert

   SOX17-T2A-dTomato-PGK-Neo-SOX17 (SOX17 Human)
   

   Use

   Cre/Lox; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-FLAG-HAND1-T2A-mTagBFP2

   Plasmid

   #223192

   Purpose

   Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

   Depositor

   Matthew Birket

   Article

   Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

   Insert

   HAND1 (HAND1 Human)
   

   Use

   Promoterless entry vector for gateway cloning

   Tags

   FLAG and T2A-mTagBFP2

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBiFC-SS-VN155(I152L)-IRE1-dLD

   Plasmid

   #217752

   Purpose

   Expresses the VN fragment of BiFC-IRE1-dLD

   Depositor

   Christina Chan

   Article

   Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

   Insert

   Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)
   

   Use

   Tags

   Venus (1-154, I152L)

   Expression

   Mammalian

   Mutation

   deleted amino acids 29-408

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-CLIP-CD28(CD45TM)

   Plasmid

   #223629

   Purpose

   Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   CLIP

   Expression

   Mutation

   CD28 transmembrane domain replaced by CD45 transm…

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-Spy-CD28(CD45TM)

   Plasmid

   #223624

   Purpose

   Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   Spytag003

   Expression

   Mutation

   CD28 transmembrane domain replaced by CD45 transm…

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57.1-Gata6-3xT7

   Plasmid

   #200889

   Purpose

   Inducible lentiviral expression of Gata6

   Depositor

   Kai Ge

   Article

   Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

   Insert

   Gata6 (Gata6 Mouse)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   3xT7

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-Hygro-Ef1A-Myc-POLB(PAMmut)

    Plasmid

    #176150

    Purpose

    N-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by POLB gRNA1

    Promoter

    EF1A

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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