We narrowed to 4,263 results for: Mcc

1. pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

   Plasmid

   #177320

   Purpose

   Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).

   Depositor

   Xiaolin Nan, Steven Chu

   Article

   Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

   Insert

   KRAS 4B (KRAS Human)
   

   Use

   Lentiviral

   Tags

   FKBP-derived dimerization domain (DD, or DmrB) an…

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   TetOn-CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-GAlpha12-RLuc8

   Plasmid

   #140985

   Purpose

   Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlpha12-RLuc8 (GNA12 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. R725-X11-566: His6-MBP-tev-Hs.LGALS3

   Plasmid

   #159699

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.LGALS3

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.LGALS3 (LGALS3 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-GFAP-eGFP-shAqp4-4

   Plasmid

   #223225

   Purpose

   mir30 based shRNA strategy, shRNA targeting mouse Aqp4

   Depositor

   Li-Huei Tsai

   Article

   Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

   Insert

   shAqp4 (Aqp4 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-GFAP-eGFP-shAqp4-3

   Plasmid

   #223224

   Purpose

   mir30 based shRNA strategy, shRNA targeting mouse Aqp4

   Depositor

   Li-Huei Tsai

   Article

   Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

   Insert

   shAqp4 (Aqp4 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-GFAP-eGFP-shAqp4-1

   Plasmid

   #223223

   Purpose

   mir30 based shRNA strategy, shRNA targeting mouse Aqp4

   Depositor

   Li-Huei Tsai

   Article

   Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

   Insert

   shAqp4 (Aqp4 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

   Plasmid

   #169160

   Purpose

   To express nsp14-nsp10 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-CMV-EGFP-PolB-PAMmut-Hygro

   Plasmid

   #176086

   Purpose

   EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Mutation in the PAM site used by PolBKOg1

   Promoter

   CMV

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tet-O-FUW-GLI1-P2A-mCherry

   Plasmid

   #203858

   Purpose

   Tet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   GLI1 (GLI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)

    Plasmid

    #159687

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)

    Depositor

    Dominic Esposito

    Article

    Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

    Insert

    His6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

    Plasmid

    #159700

    Purpose

    E. coli protein expression

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)
    

    Use

    Tags

    His6-MBP-tev-Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. VARP GFP-pLXIN

    Plasmid

    #62950

    Purpose

    full length human VARP with C-terminal EGFP tag cloned into pLXIN

    Depositor

    Paul Luzio

    Article

    Hesketh et al Dev Cell. 2014 Jun 9;29(5):591-606. doi:

    Insert

    VARP (ANKRD27 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations of amino acids 422-428 to produc…

    Promoter

    LTR

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLV-hCCND1-T286A-HA

    Plasmid

    #174155

    Purpose

    Lentiviral vector expressing HA-tagged human cyclin D1 with T286A mutation

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    A>G at base 856 (Thr>Ala at amino acid 286)

    Promoter

    EF1A

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tet-O-FUW-MAFB-P2A-mCherry

    Plasmid

    #203866

    Purpose

    Tet-inducible lentiviral plasmid expressing MAFB-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    MAFB (MAFB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107582

    Purpose

    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology; E. coli and bacillus shuttle v…

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    fba promoter Bacillus megaterium DSM319

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4-deltaH-AMBRA1-3xFLAG

    Plasmid

    #174158

    Purpose

    Vector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted)

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 1-34

    Promoter

    CMV

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.23 C3_7

    Plasmid

    #60293

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    SLC30A8 enhancer (SLC30A8 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Tet-O-FUW-KLF4-P2A-mCherry

    Plasmid

    #203855

    Purpose

    Tet-inducible lentiviral plasmid expressing KLF4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-PGK-EGFP-ALDH1A3-Neo

    Plasmid

    #189744

    Purpose

    The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A3 (ALDH1A3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. EF1a-TALEN_HD (SPC right)

    Plasmid

    #126695

    Purpose

    right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    human SPC right
    

    Use

    TALEN

    Tags

    NLS, truncated N term and TAL, FokI

    Expression

    Mammalian

    Mutation

    R451P in SPC

    Promoter

    EF1

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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