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pMK391 (mAID-BSD)

Plasmid

#121192

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-BSD

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD6

Plasmid

#73915

Purpose

tissue-specific multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

:tRNA:gRNA:tRNA:gRNA:tRNA

Use

CRISPR

Expression

Insect

Promoter

UAS-DSCP

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGH125_dCas9-Blast

Plasmid

#85417

Purpose

lentiviral expression vector for dCas9 with Blasticidin selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

dCas9 and Blasticidin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Camui-CR

Plasmid

#40256

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Camuiα-CR

Expression

Mammalian

Promoter

CMV IE

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pBAMD1-6

Plasmid

#61566

Purpose

Standardized mini-Tn5 delivery plasmid, gentamicin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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NW50

Plasmid

#133445

Purpose

Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW50

Expression

Bacterial

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP nLAP -2

Plasmid

#194322

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP nLAP -1

Plasmid

#194321

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP nLAP -0

Plasmid

#194320

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP nLAP +1

Plasmid

#194306

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP nLAP +2

Plasmid

#194307

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mRuby2-FRET-10

Plasmid

#58179

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

Type

Empty backbone

Tags

mNeonGreen and mRuby2

Expression

Mammalian

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMK392 (mAID-mClover-BSD)

Plasmid

#121193

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-mClover-BS

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Str-Ii_VSVG-SBP-mCherry

Plasmid

#65301

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Connexin43-GFP-APEX2

Plasmid

#49385

Purpose

Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Connexin43 - EGFP - APEX2

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available Since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pBAMD1-2

Plasmid

#61564

Purpose

Standardized mini-Tn5 delivery plasmid, kanamycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMK394 (mAID-Nluc-Neo)

Plasmid

#121195

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-Nluc-Neo

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

Plasmid

#31484

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

EF1s HA NLS Sce(opt) T2A IFP1.4

Use

Lentiviral

Tags

HA and NLS

Mutation

"Opt" indicates the I-SceI coding seque…

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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p70a-T7RNAP

Plasmid

#105683

Purpose

Expresses T7 polymerase in cell-free TXTL.

Depositor

Chase Beisel

Article

Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

Insert

T7 polymerase

Expression

Bacterial

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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