We narrowed to 24,923 results for: fer

1. NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

   Plasmid

   #112022

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO alpha/beta into TRBC1 HDRDT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196078

   Purpose

   (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL4.23-IL2RA CaRE4 (plus strand)

   Plasmid

   #91850

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0345)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE4 (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL4.23-IL2RA CaRE4 (minus strand)

   Plasmid

   #91849

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0626)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE4 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NYESO alpha into TRAC HDRT Source (pTR 223)

   Plasmid

   #112023

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO alpha into TRAC HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NYESO beta into TRBC1 HDRT Source (pTR 277)

   Plasmid

   #112024

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO beta into TRBC1 HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL4.23-IL2RA CaRE1 (plus strand)

   Plasmid

   #91837

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0614)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE1 (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Promoter

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCW-codon optimized catalytically inactive PHGDH

   Plasmid

   #154903

   Purpose

   Expresses codon optimized catalytically inactive PHGDH in mammalian cells

   Depositor

   Michael Pacold

   Article

   Ngo et al Cancer Discov. 2020 Sep;10(9):1352-1373.

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Three mutations: D175N, R236K, H283A

   Promoter

   TRE

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196076

   Purpose

   (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-N1 hMOG-beta2-EGFP

    Plasmid

    #160979

    Purpose

    Expresses a human myelin oligodendrocyte glycoprotein isoform beta2 - EGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

    Insert

    Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2 (MOG Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196080

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196074

    Purpose

    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL4.23-IL2RA CaRE5 (plus strand)

    Plasmid

    #91841

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0618)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE5 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL4.23-IL2RA CaRE5 (minus strand)

    Plasmid

    #91836

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0613)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE5 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAdeasy-CMV-AE-EF1α-R2

    Plasmid

    #194928

    Purpose

    expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis

    Depositor

    Limei Wang

    Article

    Wang et al Vaccines (Basel). 2024 Sep 6;12(9):1022.

    Inserts

    AE
    EEF1A1 (EEF1A1 Human)
    R2
    

    Use

    Adenoviral

    Tags

    Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c

    Expression

    Mutation

    a 48 bp linker (Gly3ser)4 was added between the t…

    Promoter

    CMV and EF1a

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL4.23-IL2RA CaRE1 (minus strand)

    Plasmid

    #91838

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0615)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE1 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RAB11A-GFP HDRT Source (pTR 143)

    Plasmid

    #112012

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-GFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RAB11A-mCherry HDRT Source (pTR 144)

    Plasmid

    #112013

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-mCherry HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CD4-C-GFP HDRT Source (pTR 152)

    Plasmid

    #112018

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-GFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118155

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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