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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,437 results for: hells

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Showing: 2321 - 2340 of 2437 results

  7. pDY118A

    Plasmid
    #182958
    Purpose
    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Inserts
    Cas9
    Gam, Beta, Exo
    I-scel
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182958-plasmid-map-sequence-id-365360

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  10. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  11. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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  18. pIFEX-A2aGH

    Plasmid
    #160542
    Purpose
    pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Expression
    Yeast
    Mutation
    N/A
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160542-plasmid-map-sequence-id-317791

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  19. pEFEX1-A2aGH

    Plasmid
    #160543
    Purpose
    pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Expression
    Yeast
    Mutation
    N/A
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160543-plasmid-map-sequence-id-317760

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  20. pEFEX2-A2aGH

    Plasmid
    #160544
    Purpose
    pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Expression
    Yeast
    Mutation
    N/A
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160544-plasmid-map-sequence-id-317794

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Showing: 2321 - 2340 of 2437 results