We narrowed to 4,370 results for: p2a

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Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

Plasmid

#231910

Purpose

For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

Plasmid

#228400

Purpose

Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NES/PKI-mNeonGreen2x-NLS/FUS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro

Plasmid

#228398

Purpose

Encodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NLS/cMyc-AcGFP2x-NES/iKB2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo

Plasmid

#222637

Purpose

Ezrin activation reporter, T567 phosphorylation deficient

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

miRFP680-NES-p2A-ezrin-T567A-mRuby3 (EZR Human)

Use

Lentiviral

Tags

mRuby3

Mutation

T567A

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

Plasmid

#187332

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT257 Lenti pEF-dCas9-3XFLAG-Z+F-T2A-tagBFP-P2A-Blast

Plasmid

#187333

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-Z+F+N-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT255 Lenti pEF-dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast

Plasmid

#187331

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2-nbt300 2n-KalNFB-3xmir1T-syt2a-10U2n-mCardinal-P2A-mClover3

Plasmid

#196117

Purpose

zebrafish expression

Depositor

Reinhard Köster

Insert

nbt300 2n-KalNFB-3xmir1T-syt2a-HO1-10U2n-mCardinal-P2A-mClover3

Use

Zebrafish expression

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pW228-lenti-sg1-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189944

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg1-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #1

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW229-lenti-sg2-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189945

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg2-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #2

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW230-lenti-sg3-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189946

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg3-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #3

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW317-lenti-sg1-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189947

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg1-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #1

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW318-lenti-sg2-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189948

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg2-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #2

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW319-lenti-sg3-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189949

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg3-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #3

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW401-lenti-sg1-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189950

Purpose

Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg1-Itgb1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #1

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW402-lenti-sg2-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189951

Purpose

Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg2-Itgb1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #2

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW403-lenti-sg3-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189952

Purpose

Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg3-Itgb1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #3

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

Plasmid

#185489

Purpose

CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

Depositor

Benjamin Kleinstiver, Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-eGFP

Expression

Mammalian

Mutation

H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…

Promoter

CMV and T7

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

Plasmid

#185492

Purpose

CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

Depositor

Benjamin Kleinstiver, Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-eGFP

Expression

Mammalian

Mutation

D10A

Promoter

CMV and T7

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro

Plasmid

#174118

Purpose

Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro

Use

Lentiviral

Promoter

mU6 and UbC

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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