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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,093 results for: sgRNA

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Showing: 2321 - 2340 of 9093 results

  1. IABE

    Plasmid
    #247332
    Purpose
    Vector encoding human codon-optimized engineered DelIscB nickase fusion with TadA8e driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB(D60A)-2×TadA8e(V106W)_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247332-plasmid-map-sequence-id-496501

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  12. pRS159

    Plasmid
    #122378
    Purpose
    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122378-plasmid-map-sequence-id-241243

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  13. pCB-2

    Plasmid
    #210388
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available Since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210388-plasmid-map-sequence-id-418474

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  14. pEditC

    Plasmid
    #207532
    Purpose
    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Available Since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207532-plasmid-map-sequence-id-409609

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  15. pEditA-RY

    Plasmid
    #207533
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Available Since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207533-plasmid-map-sequence-id-409612

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  16. pEditC-RY

    Plasmid
    #207534
    Purpose
    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Available Since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207534-plasmid-map-sequence-id-409613

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  19. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181781-plasmid-map-sequence-id-354678

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Showing: 2321 - 2340 of 9093 results