We narrowed to 8,835 results for: sgRNA

1. pGuide-P1-O1a (EL702)

   Plasmid

   #140040

   Purpose

   CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

   Depositor

   Johan Elf

   Article

   Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

   Insert

   barcode P1 and sgRNA lacO1 array
   

   Use

   Synthetic Biology

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-hsg(NT:NT)

   Plasmid

   #138260

   Purpose

   Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   Nontargeting sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgPYM1

   Plasmid

   #105249

   Purpose

   human PYM1 sgRNA

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

   Insert

   sgRNA for PYM1
   

   Expression

   Mammalian

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pENTR223-CMV-Cre-U6-sgX-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

   Plasmid

   #209060

   Purpose

   Entry cloning vector to insert an sgRNA of interest (using Esp3i digestion) into a vector that already contains sgRNAs against mouse Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

   Depositor

   Matthew Oser

   Article

   Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

   Insert

   sgRNAs targeting Rb1, Trp53, Rbl2 and Cre recombinase
   

   Use

   Gateway vector to be used for lr reaction

   Promoter

   U6

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXPR_045

   Plasmid

   #107144

   Purpose

   cloning of a sgRNA

   Depositor

   David Root

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1-a and hU6

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLib6.6

   Plasmid

   #176652

   Purpose

   Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEditA

   Plasmid

   #207531

   Purpose

   Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
   

   Use

   CRISPR; Pseudomonas vector

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LRCherry2.1

   Plasmid

   #108099

   Purpose

   Lentiviral expression plasmid of sgRNA with mCherry

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CMV-NLS-dCas9-NLS

    Plasmid

    #99908

    Purpose

    For the expression of NLS-dCas9-NLS

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Insert

    NLS-dCas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRG2.1

    Plasmid

    #108098

    Purpose

    Lentiviral expression plasmid of sgRNA with GFP

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-EN1201

    Plasmid

    #92144

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDIV270

    Plasmid

    #226181

    Purpose

    Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    TEF1p (Komagataella phaffii)

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGCP123-EbpA_g1

    Plasmid

    #153517

    Purpose

    sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LRG2.1_Puro

    Plasmid

    #125594

    Purpose

    Lentiviral expression of sgRNA with GFP and puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pV1382

    Plasmid

    #111436

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAT009

    Plasmid

    #171635

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sg-A

    Plasmid

    #83807

    Purpose

    Express sgRNA targeting SA site

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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