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pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Tags

His

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-mTagBFP-CAAX

Plasmid

#75176

Purpose

Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP-CAAX

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMVV207

Plasmid

#215343

Purpose

gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

S. stutzeri nifL gRNA

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

J23119

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-VEGFR2-225

Plasmid

#21306

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pET28b-CoilR-Cys

Plasmid

#181989

Purpose

Used to label proteins with the CoilE genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

CoilR-Cys

Tags

His-Tag

Expression

Bacterial

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCLC3sHA

Plasmid

#52424

Purpose

mammalian expression of HA tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Wang et al Gastroenterology. 2004 Apr;126(4):1157-6

Insert

CLC3 (Clcn3 Rat)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-TTF-1-wt

Plasmid

#74990

Purpose

Retroviral vector holding the human wild-type full-length TTF-1 (NKX2-1) cDNA

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 (TTF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

wild-type, full-length, without 3' UTR

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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Integrin beta1(1-455)-tev-BHCys

Plasmid

#27315

Depositor

Timothy Springer

Article

Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

Insert

integrin beta1 (ITGB1 Human)

Expression

Mammalian

Mutation

beta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only
pPbuCas13b

Plasmid

#184565

Purpose

Inducible expression of PbuCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Cas13b nuclease from Prevotella buccae

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pXWJ109Hg-gfp

Plasmid

#123148

Purpose

A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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