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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,994 results for: ALP

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Showing: 2341 - 2360 of 7994 results

  1. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

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  2. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  3. TRUPATH Triple Gagust

    Plasmid
    #196054
    Purpose
    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196054-plasmid-map-sequence-id-393090

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  4. TRUPATH

    Plasmid Kit
    #1000000163
    Purpose
    BRET biosensor plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation.
    Depositor
    Bryan Roth
    Application
    Gene Expression and Labeling
    Vector Type
    Mammalian Expression
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only

  5. TFORF2975

    Plasmid
    #144451
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NAT10 (NAT10 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144451-plasmid-map-sequence-id-407333

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  6. TFORF2510

    Plasmid
    #144032
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NAT10 (NAT10 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144032-plasmid-map-sequence-id-400534

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  10. PB513B-1/TRE-hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ-EF1α-Puro

    Plasmid
    #199550
    Purpose
    PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBP-γ) under control of the TRE/PCMVmin promoter
    Depositor
    Masamichi Kamihira
    Article
    Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33
    Insert
    hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ (HNF1A, FOXA2, CEBPB, CEBPA, CEBPG Human)
    Use
    Piggybac transposon vector
    Expression
    Mammalian
    Promoter
    TRE+CMVmin promoter
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    199550-plasmid-map-sequence-id-405449

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  11. GoldenBraid 2.0 Kit

    Plasmid Kit
    #1000000076
    Purpose
    Plant synthetic biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts.
    Depositor
    Diego Orzaez
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Plant Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate (GoldenBraid)
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. ONE-GO biosensors

    Plasmid Kit
    #1000000224
    Purpose
    One-vector G protein Optical (ONE-GO) biosensor plasmids for the expression of BRET-based biosensors of G proteins activated by GPCRs.
    Depositor
    Mikel Garcia-Marcos
    Application
    Gene Expression and Labeling
    Vector Type
    Lentiviral, Mammalian Expression
    Available Since
    Jan. 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pHIV-Luc-ZsGreen

    Plasmid
    #39196
    Depositor
    Bryan Welm
    Inserts
    Firefly Luciferase Luc2P
    ZsGreen
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-alpha and EF1-alpha
    Available Since
    Oct. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    39196-plasmid-map-sequence-id-335121

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  14. pHIV-Luciferase

    Plasmid
    #21375
    Purpose
    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase
    Depositor
    Bryan Welm
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    21375-plasmid-map-sequence-id-357945

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  15. Gaq-LSS-SGFP2

    Plasmid
    #112951
    Purpose
    Galphaq tagged with LSS-SGFP2
    Depositor
    Dorus Gadella
    Insert
    Galphaq
    Tags
    LSS-SGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    112951-plasmid-map-sequence-id-221967

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  16. pEYFP-C1-p85beta

    Plasmid
    #1408
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p85beta (Pik3r2 Mouse)
    Tags
    eYFP
    Expression
    Mammalian
    Available Since
    June 22, 2006
    Availability
    Academic Institutions and Nonprofits only

  17. pRSV-PKI-v2

    Plasmid
    #45066
    Purpose
    Expression of PKI (PKA inhibitor)
    Depositor
    Richard Maurer
    Insert
    cAMP-dependent protein kinase inhibitor alpha
    Expression
    Mammalian
    Promoter
    RSV
    Available Since
    July 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    45066-plasmid-map-sequence-id-62500

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  18. pGEX-4T3-p85beta

    Plasmid
    #1406
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p85beta (Pik3r2 Mouse)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only

  19. pRSV-PKImut-v2

    Plasmid
    #45067
    Purpose
    Expression of intactive PKI (PKA inhibitor) mutant
    Depositor
    Richard Maurer
    Insert
    cAMP-dependent protein kinase inhibitor alpha
    Expression
    Mammalian
    Mutation
    changed Arg 20 & 21 to Glycines
    Promoter
    RSV
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    45067-plasmid-map-sequence-id-62502

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  20. pHCA/GAL4(848).ER

    Plasmid
    #108215
    Purpose
    Yeast expression vector for fusion of Gal4 AA 1-848 to hormone binding domain of estrogen receptor a
    Depositor
    Didier Picard
    Insert
    Gal4-ERalpha HBD
    Expression
    Yeast
    Mutation
    ERalpha HBD has G400V mutation
    Promoter
    ADH0
    Available Since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108215-plasmid-map-sequence-id-265031

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Showing: 2341 - 2360 of 7994 results