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We narrowed to 2,493 results for: D10

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Showing: 2341 - 2360 of 2493 results

  2. SpCas9-miniCGBE1

    Plasmid
    #198548
    Purpose
    Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198548-plasmid-map-sequence-id-388891

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  5. pSCBE3-NG-HF1

    Plasmid
    #218160
    Purpose
    This plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218160-plasmid-map-sequence-id-433202

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  6. pSCBE3-NG-Hypa

    Plasmid
    #218161
    Purpose
    This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N692A…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218161-plasmid-map-sequence-id-433271

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  7. pSCBE3-HF1-Hypa

    Plasmid
    #218158
    Purpose
    This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218158-plasmid-map-sequence-id-433272

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  11. pD649-HAsp-ICAM2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156610
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Publication
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    ICAM2 (ICAM2 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156610-plasmid-map-sequence-id-302329

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  19. pFLAG-CMV2-mMLL4fusion

    Plasmid
    #256014
    Purpose
    This plasmid expresses a mutant mini-version of human MLL4 (KMT2D) composed of a mutant PHD4-6 (1358–1572aa) bearing E1516A/E1517A/D1518A/E1544A and a SET-containing C-terminal region (4507–5537aa).
    Depositor
    Min Gyu Lee
    Publication
    Dhar et al Genes Dev. 2012 Dec 15;26(24):2749-62. d
    Insert
    mMLL4 fusion (KMT2D Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    PHD(PHD4-6) and SET(Catalytic domain) along with …
    Promoter
    CMV
    Available since
    June 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    256014-plasmid-map-sequence-id-513256

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  20. pFLAG-CMV2-MLL4fusion

    Plasmid
    #255997
    Purpose
    This plasmid expresses a mini-version of human MLL4 (aka KMT2D), which is composed of a tandem PHD (PHD4-6;1358–1572aa) and a SET (catalytic domain)-containing C-terminal region (4507–5537aa).
    Depositor
    Min Gyu Lee
    Publication
    Dhar et al Genes Dev. 2012 Dec 15;26(24):2749-62. d
    Insert
    MLL4 fusion (KMT2D Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    MLL4fusion with PHD(4-6) 1358–1572 and SET (catal…
    Promoter
    CMV
    Available since
    June 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    255997-plasmid-map-sequence-id-513255

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Showing: 2341 - 2360 of 2493 results